| http://purl.uniprot.org/citations/9126987 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9126987 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9126987 | http://www.w3.org/2000/01/rdf-schema#comment | "A protein that binds the intracellular domain of the type 1 TNFR (TNFR-1IC) has been identified by two-hybrid cloning. The 97-kDa TNFR-associated protein, TRAP2, shows sequence identity with internal amino acid sequences from subunit 2 of the 26S protease. TRAP2 antiserum recognizes subunit 2 of the 26S protease, which is consistent with the identity of these proteins. TRAP2 antiserum interacted with the 97-kDa protein in HeLa cell lysates and cytosol, the latter observation showing that TRAP2 resides in the same cellular compartment as TNFR-1IC. A fusion of glutathione-S-transferase and TNFR-1IC (GST-TNFR-1IC) precipitated TRAP2 from a HeLa cell lysate; conversely, GST-TRAP2 precipitated TNFR-1 from such a lysate. These observations show that the proteins interact in the cellular milieu. After in vitro transcription/translation and 35S labeling, TRAP2 was precipitated from a cellfree system by GST-TNFR-1IC, showing that TNFR-1IC and TRAP2 interact directly. TRAP2 was also precipitated from the cellfree translation system by a GST fusion containing the N-terminal half of TNFR-1IC, but not by a GST fusion containing the C-terminal half of TNFR-1IC that contains a "death domain" that plays an obligatory role in signaling cytotoxicity. The ability of deletion mutants of TNFR-1IC to interact with TRAP2 was tested using the two-hybrid system. This also showed that the amino acid sequences that mediate binding reside outside of the death domain in TNFR-1IC. The demonstration that a subunit of the 26S protease binds TNFR-1 may identify a novel TNF-signaling pathway."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Guo D."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Guo D."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Song H.Y."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Song H.Y."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Dunbar J.D."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Dunbar J.D."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Donner D.B."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Donner D.B."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Wu L.-W."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/author | "Wu L.-W."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/name | "J. Immunol."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/pages | "4252-4259"xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/pages | "4252-4259"xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/title | "Two-hybrid cloning of a gene encoding TNF receptor-associated protein 2, a protein that interacts with the intracellular domain of the type 1 TNF receptor: identity with subunit 2 of the 26S protease."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/title | "Two-hybrid cloning of a gene encoding TNF receptor-associated protein 2, a protein that interacts with the intracellular domain of the type 1 TNF receptor: identity with subunit 2 of the 26S protease."xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/volume | "158"xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://purl.uniprot.org/core/volume | "158"xsd:string |
| http://purl.uniprot.org/citations/9126987 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9126987 |
| http://purl.uniprot.org/citations/9126987 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9126987 |