http://purl.uniprot.org/citations/9133737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9133737 | http://www.w3.org/2000/01/rdf-schema#comment | "Increased production of secreted proteins in Saccharomyces cerevisiae was achieved by overexpressing the yeast syntaxins. Sso1 or Sso2 protein, the t-SNAREs functioning at the targeting/fusion of the Golgi-derived secretory vesicles to the plasma membrane. Up to four- or six-fold yields of a heterologous secreted protein, Bacillus alpha-amylase, or an endogenous secreted protein, invertase, were obtained respectively when expressing either one of the SSO genes, SSO1 or SSO2, from the ADH1 promoter on a multicopy plasmid. Direct correlation between the Sso protein level and the amount of secreted alpha-amylase was demonstrated by modulating the expression level of the SSO2 gene. Quantitation of the alpha-amylase activity in the culture medium, periplasmic space and cytoplasm suggests that secretion into the periplasmic space is the primary stage at which the SSO genes exert the secretion-enhancing function. Pulse-chase data also support enhanced secretion efficiently obtained by SSO overexpression. Our data suggest that the Sso proteins may be rate-limiting components of the protein secretion machinery at the exocytosis step in yeast."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1097-0061(19970330)13:4<337::aid-yea98>3.0.co;2-k"xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Ruohonen L."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Keranen S."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Toikkanen J."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Soderlund H."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Tieaho V."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/author | "Outola M."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/name | "Yeast"xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/pages | "337-351"xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/title | "Enhancement of protein secretion in Saccharomyces cerevisiae by overproduction of Sso protein, a late-acting component of the secretory machinery."xsd:string |
http://purl.uniprot.org/citations/9133737 | http://purl.uniprot.org/core/volume | "13"xsd:string |
http://purl.uniprot.org/citations/9133737 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9133737 |
http://purl.uniprot.org/citations/9133737 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9133737 |
http://purl.uniprot.org/uniprot/#_P39926-mappedCitation-9133737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9133737 |
http://purl.uniprot.org/uniprot/#_P32867-mappedCitation-9133737 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9133737 |
http://purl.uniprot.org/uniprot/P32867 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9133737 |
http://purl.uniprot.org/uniprot/P39926 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9133737 |