| http://purl.uniprot.org/citations/9142215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9142215 | http://www.w3.org/2000/01/rdf-schema#comment | "The 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat leukemia model enables scientists to analyze cells altered by carcinogens at various stages of leukemogenesis. We have reported that a consistent type of point mutation. A-->T transversion at the second base in codon 61 of the N-ras gene, was present in this leukemia and that this mutation appeared in bone marrow cells as early as 48 h after a single dose of DMBA. In addition, two leukemia cell lines with the N-ras mutation had no wild-type N-ras allele. Therefore, we examined whether these alterations were essential to the DMBA-induced leukemias. In the study reported here, we confirmed the occurrence of this N-ras mutation in 18 (86%) of 21 primary leukemias and loss of the N-ras wild-type allele in 12 (67%) of 18 leukemias with the mutated N-ras. By using microsatellite markers on chromosome 2, loss of heterozygosity (LOH) at the N-ras locus was observed in eight leukemias, all of which were shown to have lost the wild-type N-ras allele by mutant-allele-specific amplification. These results suggest that LOH related to loss of the wild-type N-ras allele reproducibly occurs in leukemias with the N-ras mutation. Considering the timing of the N-ras mutation and LOH, it is likely that the N-ras mutation is induced early, and cells that have lost the wild-type N-ras allele seem to develop into leukemia. We believe that this system provides a suitable model for studying a series of genetic alterations from the earliest stage of carcinogenesis that cannot be approached in human malignancies."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1098-2744(199704)18:4<206::aid-mc4>3.3.co;2-j"xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/author | "Sugiyama T."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/author | "Matsuo S."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/author | "Osaka M."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/author | "Koh T."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/name | "Mol Carcinog"xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/pages | "206-212"xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/title | "Loss of heterozygosity at the N-ras locus in 7,12-dimethylbenz[a] anthracene-induced rat leukemia."xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/9142215 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9142215 |
| http://purl.uniprot.org/citations/9142215 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9142215 |
| http://purl.uniprot.org/uniprot/#_A0A8L2QIP9-mappedCitation-9142215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9142215 |
| http://purl.uniprot.org/uniprot/#_Q04970-mappedCitation-9142215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9142215 |
| http://purl.uniprot.org/uniprot/Q04970 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9142215 |
| http://purl.uniprot.org/uniprot/A0A8L2QIP9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9142215 |