| http://purl.uniprot.org/citations/9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9144214 | http://www.w3.org/2000/01/rdf-schema#comment | "Insights into the function of a gene can be gained in multiple ways, including loss-of-function phenotype, sequence similarity, expression pattern, and by the consequences of its misexpression. Analysis of the phenotypes produced by expression of a gene at an abnormal time, place, or level may provide clues to a gene's function when other approaches are not illuminating. Here we report that an eye-specific, enhancer-promoter present in the P element expression vector pGMR is able to drive high level expression in the eye of genes near the site of P element insertion. Cell fate determination, differentiation, proliferation, and death are essential for normal eye development. Thus the ability to carry out eye-specific misexpression of a significant fraction of genes in the genome, given the dispensability of the eye for viability and fertility of the adult, should provide a powerful approach for identifying regulators of these processes. To test this idea we carried out two overexpression screens for genes that function to regulate cell death. We screened for insertion-dependent dominant phenotypes in a wild-type background, and for dominant modifiers of a reaper overexpression-induced small eye phenotype. Multiple chromosomal loci were identified, including an insertion 5' to hid, a potent inducer of apoptosis, and insertions 5' to DIAP1, a cell death suppressor. To facilitate the cloning of genes near the P element insertion new misexpression vectors were created. A screen with one of these vectors identified eagle as a suppressor of a rough eye phenotype associated with overexpression of an activated Ras1 gene."xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.94.10.5195"xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/author | "Rubin G.M."xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/author | "Hay B.A."xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/author | "Maile R."xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/pages | "5195-5200"xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/title | "P element insertion-dependent gene activation in the Drosophila eye."xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://purl.uniprot.org/core/volume | "94"xsd:string |
| http://purl.uniprot.org/citations/9144214 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9144214 |
| http://purl.uniprot.org/citations/9144214 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9144214 |
| http://purl.uniprot.org/uniprot/#_P15370-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KGX3-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_P10090-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_P13360-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_M9PFL5-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_Q24106-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_Q24475-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_M9PI74-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_M9PIJ7-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_P08646-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_Q24306-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |
| http://purl.uniprot.org/uniprot/#_Q8MSC5-mappedCitation-9144214 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9144214 |