| http://purl.uniprot.org/citations/9147053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9147053 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9147053 | http://www.w3.org/2000/01/rdf-schema#comment | "As part of our ongoing efforts to characterize the N-glycosylation pathway of lepidopteran insect cells, we have isolated an alpha 1,2-mannosidase homolog from an Sf9 cDNA library. This cDNA contains an open reading frame which encodes a 670 amino acid protein with a calculated molecular weight of 75,225 Da. This protein has two potential N-glycosylation sites, two consensus calcium binding sequences, and is predicted to be a type II integral membrane protein with a 22 amino acid transmembrane domain (residues 31-52). The amino acid sequence of this protein is 35-57% identical to Drosophila, human, murine, and yeast alpha 1,2-mannosidases. A transcript of approximately 6 kilobases was detected by Northern blot analysis of Sf9 mRNA. Genomic Southern blots probed with an intron-free fragment of the alpha 1,2-mannosidase gene indicated that there are at least two copies or cross-hybridizing variants of this gene in the Sf9 genome. In vivo expression of the cDNA using a recombinant baculovirus produced a protein that released [3H]mannose from [3H]Man9GlcNAc. This activity required calcium, but not magnesium, and was inhibited by 1-deoxymannojirimycin. These results indicate that Sf9 cells encode and express an alpha 1,2-mannosidase with properties similar to those of other eukaryotic processing alpha 1,2-mannosidases."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/7.3.433"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/7.3.433"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Jarvis D.L."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Jarvis D.L."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Herscovics A."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Herscovics A."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Kawar Z."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/author | "Kawar Z."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/pages | "433-443"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/pages | "433-443"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/title | "Isolation and characterization of an alpha 1,2-mannosidase cDNA from the lepidopteran insect cell line Sf9."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/title | "Isolation and characterization of an alpha 1,2-mannosidase cDNA from the lepidopteran insect cell line Sf9."xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/9147053 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9147053 |
| http://purl.uniprot.org/citations/9147053 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9147053 |
| http://purl.uniprot.org/citations/9147053 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9147053 |
| http://purl.uniprot.org/citations/9147053 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9147053 |