| http://purl.uniprot.org/citations/9150941 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9150941 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9150941 | http://www.w3.org/2000/01/rdf-schema#comment | "Employing isoelectric focusing on immobilized pH gradients followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) we have obtained a map of C. elegans proteins, from a mixed culture containing all developmental stages, presenting over 2000 spots within the window of isoelectric points (pI) 3.5-9 and a molecular mass of 10-200 kDa. Edman microsequencing yielded successful results in 12 out of 24 analyzed spots. All but one of the N-terminal sequences retrieved C. elegans sequences in cosmid and/or expressed sequence tag clones. Structurally related protein sequences found in data banks included enzymes in energy metabolism (cytochrome oxydase, ATP synthase, enolase), a fatty acid-binding protein, a translationally controlled tumor protein, an unknown C. elegans protein, an acidic ribosomal protein, a titin-like protein, a G-protein beta chain, cyclophilin, and cathepsin D. Experimental determination of N-termini allowed us to define sites of signal cleavage providing further information on the physiological role of the newly found C. elegans proteins. This report demonstrates the possibility of two-dimensional gel electrophoresis and Edman microsequencing in the elucidation of C. elegans proteome."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.org/dc/terms/identifier | "doi:10.1002/elps.1150180337"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.org/dc/terms/identifier | "doi:10.1002/elps.1150180337"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Heid H."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Heid H."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Bini L."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Bini L."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Pallini V."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Pallini V."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Liberatori S."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Liberatori S."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Geier G."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Geier G."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Zwilling R."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/author | "Zwilling R."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/name | "Electrophoresis"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/pages | "557-562"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/pages | "557-562"xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/title | "Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing."xsd:string |
| http://purl.uniprot.org/citations/9150941 | http://purl.uniprot.org/core/title | "Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing."xsd:string |