| http://purl.uniprot.org/citations/9154926 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9154926 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9154926 | http://www.w3.org/2000/01/rdf-schema#comment | "Bovine milk fat globule membranes are a rich source of glycoproteins PAS-6 (52 kDa) and PAS-7 (47 kDa). They are glycosylation variants sharing a common polypeptide core. The PAS-6/7 protein consists of two EGF-like domains and a tandem repeated structure with a high degree of similarity to the C1 and C2 domains found in blood-clotting factors V and VIII. The second EGF-like domain contains an RGD cell adhesion sequence with the possibility of binding integrins, while the C-terminal end of the C2-like domain contains a probable amphipathic alpha-helix. Using a PAS-6/7 column, bovine alpha v beta 5 integrin was purified from mammary gland tissue by affinity chromatography and characterized by Western blotting and N-terminal sequencing. The interaction between PAS-6/7 and the alpha v beta 5 integrin was shown to be RGD dependent. Lipid binding assays showed that PAS-6/7 binds to surfaces of phosphatidylserine, -inositol, and -glycerol, and their precursor, phosphatidic acid, but not phosphatidylcholine. Furthermore, PAS-6/7 displayed the highest affinity toward a total lipid fraction derived from the milk fat globule membrane as compared to pure phospholipids. Using Western blotting technique, PAS-6/7 was shown to be widely expressed in a number of tissues. These results show that PAS-6/7 is a common protein which can bind to membranes by two distinct mechanisms, one through affinity to integrin alpha v beta 5 and another by direct binding to phospholipids."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi963119m"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi963119m"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Berglund L."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Berglund L."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Petersen T.E."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Petersen T.E."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Andersen M.H."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Andersen M.H."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Rasmussen J.T."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/author | "Rasmussen J.T."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/pages | "5441-5446"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/pages | "5441-5446"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/title | "Bovine PAS-6/7 binds alpha v beta 5 integrins and anionic phospholipids through two domains."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/title | "Bovine PAS-6/7 binds alpha v beta 5 integrins and anionic phospholipids through two domains."xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/volume | "36"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://purl.uniprot.org/core/volume | "36"xsd:string |
| http://purl.uniprot.org/citations/9154926 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9154926 |
| http://purl.uniprot.org/citations/9154926 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9154926 |