| http://purl.uniprot.org/citations/9162045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9162045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9162045 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9162045 | http://www.w3.org/2000/01/rdf-schema#comment | "Adipose tissue is an important storage depot for retinol, but there are no data regarding retinol mobilization from adipose stores. To address this, dibutyryl cAMP was provided to murine BFC-1beta adipocytes and its effects on retinol efflux assessed. High performance liquid chromatography analysis of retinol and retinyl esters in adipocytes and media indicated that cAMP stimulated, in a time- and dose-dependent manner, retinol accumulation in the culture media and decreased cellular retinyl ester concentrations. Study of adipocyte retinol-binding protein synthesis and secretion indicated that cAMP-stimulated retinol efflux into the media did not result from increased retinol-retinol-binding protein secretion but was dependent on the presence of fetal bovine serum in the culture media. Since our data suggested that retinyl esters can be hydrolyzed by a cAMP-dependent enzyme like hormone-sensitive lipase (HSL), in separate studies, we purified a HSL-containing fraction from BFC-1beta adipocytes and demonstrated that it catalyzed retinyl palmitate hydrolysis. Homogenates of Chinese hamster ovary cells overexpressing HSL catalyzed retinyl palmitate hydrolysis in a time-, protein-, and substrate-dependent manner, with an apparent Km for retinyl palmitate of 161 microM, whereas homogenates from control Chinese hamster ovary cells did not."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.22.14159"xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.22.14159"xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Patel S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Patel S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Shen H."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Shen H."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Wei S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Wei S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Kraemer F.B."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Kraemer F.B."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Blaner W.S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Blaner W.S."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Lai K."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Lai K."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Colantuoni V."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Colantuoni V."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Piantedosi R."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/author | "Piantedosi R."xsd:string |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9162045 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |