| http://purl.uniprot.org/citations/9165006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9165006 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9165006 | http://www.w3.org/2000/01/rdf-schema#comment | "Vitamin D-24-hydroxylase (24-OHase) is a cytochrome P-450 enzyme that catalyzes the conversion of 25-hydroxyvitamin D3 (25OHD3) and 1alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] to 24,25-dihydroxyvitamin D3 and 1,24,25-trihydroxyvitamin D3, respectively. A full-length complementary DNA for mouse 24-OHase has now been characterized. The complementary DNA consists of 3309 bp and encodes a protein of 514 amino acids that shows 82% and 95% sequence identity with the human and rat enzymes, respectively. Northern blot analysis of tissues from mice injected with 1,25-(OH)2D3 (24 pmol/g) revealed that the 3.4-kb 24-OHase messenger RNA (mRNA) is most abundant in kidney and intestine, with smaller amounts present in skin, thymus, and bone. RT-PCR and Southern blot analysis detected 24-OHase mRNA in several other tissues including lung, testis, spleen, pancreas, and heart. Intraperitoneal injection of 1,25-(OH)2D3 induced dose- and time-dependent increases in both 24-OHase mRNA abundance and enzyme activity in mouse kidney. Similarly, 1,25-(OH)2D3-induced increases in both 24-OHase mRNA and activity were apparent in the duodenum. Although 1,25-(OH)2D3 increased the amount of 24-OHase mRNA in skin, enzyme activity was not detected in this tissue. Pretreatment of mice with cycloheximide (400 microg/g), an inhibitor of protein synthesis, potentiated the increase in 24-OHase mRNA abundance, but blocked the increase in 24-OHase activity, induced by 1,25-(OH)2D3 in kidney and duodenum, suggesting that 24-OHase gene expression may be regulated not only by the vitamin D receptor but also by a short-lived repressor protein."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.138.6.5170"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.org/dc/terms/identifier | "doi:10.1210/endo.138.6.5170"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Akeno N."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Akeno N."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Horiuchi N."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Horiuchi N."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Kawane T."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Kawane T."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Saikatsu S."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/author | "Saikatsu S."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/name | "Endocrinology"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/pages | "2233-2240"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/pages | "2233-2240"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/title | "Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/title | "Mouse vitamin D-24-hydroxylase: molecular cloning, tissue distribution, and transcriptional regulation by 1alpha,25-dihydroxyvitamin D3."xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/9165006 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9165006 |
| http://purl.uniprot.org/citations/9165006 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9165006 |