| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/2000/01/rdf-schema#comment | "Dihydroneopterin triphosphate (H2NTP) 2'-epimerase from Escherichia coli catalyzes the epimerization of H2NTP to dihydromonapterin triphosphate (H2MTP). The enzyme was purified 954-fold to apparent homogeneity by a combination of ammonium sulfate fractionation and column chromatography of Cibacron blue 3GA dye ligand, phenyl-Sepharose CL-4B, methotrexate-agarose, and Superdex 200 HR 10/30 FPLC column. The molecular mass of the epimerase determined on a Superdex column was 82.6 kDa, while the subunit molecular mass determined on SDS-polyacrylamide gel electrophoresis was 13.7 kDa. This implies that the epimerase most probably exists as homohexamer. The 20-amino acid sequence from the N terminus was determined (AQPAAIIRIKNLRLRTFIGI). Based on this sequence, the gene encoding the epimerase was cloned using a simple polymerase chain reaction approach. Translation of the nucleotide sequence of the cloned gene revealed the presence of an open reading frame containing 120 amino acids with a predicted molecular mass of 13,993 Da. The epimerase gene located in a 2.3-kilobase BamHI-EcoRI fragment from Kohara's clone 406 was overexpressed 300-fold, which was confirmed by the prominent increase in the 14-kDa protein band on SDS-polyacrylamide electrophoresis gels. It showed no homology with the sequences of isomerases or other enzymes in GenBank/EMBL data bases."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.24.15323"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.24.15323"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Yim J."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Yim J."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Ahn C."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Ahn C."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Byun J."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/author | "Byun J."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/pages | "15323-15328"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/pages | "15323-15328"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/title | "Purification, cloning, and functional expression of dihydroneopterin triphosphate 2'-epimerase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/title | "Purification, cloning, and functional expression of dihydroneopterin triphosphate 2'-epimerase from Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9182560 |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9182560 |
| http://purl.uniprot.org/citations/9182560 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9182560 |