| http://purl.uniprot.org/citations/9218461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9218461 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9218461 | http://www.w3.org/2000/01/rdf-schema#comment | "The rapid movement of phospholipids (PL) between plasma membrane leaflets in response to increased intracellular Ca2+ is thought to play a key role in expression of platelet procoagulant activity and in clearance of injured or apoptotic cells. We recently reported isolation of a approximately 37-kDa protein in erythrocyte membrane that mediates Ca2+-dependent movement of PL between membrane leaflets, similar to that observed upon elevation of Ca2+ in the cytosol (Bassé, F., Stout, J. G., Sims, P. J., and Wiedmer, T. (1996) J. Biol. Chem. 271, 17205-17210). Based on internal peptide sequence obtained from this protein, a 1,445-base pair cDNA was cloned from a K-562 cDNA library. The deduced "PL scramblase" protein is a proline-rich, type II plasma membrane protein with a single transmembrane segment near the C terminus. Antibody against the deduced C-terminal peptide was found to precipitate the approximately 37-kDa red blood cell protein and absorb PL scramblase activity, confirming the identity of the cloned cDNA to erythrocyte PL scramblase. Ca2+-dependent PL scramblase activity was also demonstrated in recombinant protein expressed from plasmid containing the cDNA. Quantitative immunoblotting revealed an approximately 10-fold higher abundance of PL scramblase in platelet ( approximately 10(4) molecules/cell) than in erythrocyte ( approximately 10(3) molecules/cell), consistent with apparent increased PL scramblase activity of the platelet plasma membrane. PL scramblase mRNA was found in a variety of hematologic and nonhematologic cells and tissues, suggesting that this protein functions in all cells."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.29.18240"xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.29.18240"xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Zhou Q."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Zhou Q."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Sims P.J."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Sims P.J."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Wiedmer T."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Wiedmer T."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Stout J.G."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Stout J.G."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Luhm R.A."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/author | "Luhm R.A."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/pages | "18240-18244"xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/pages | "18240-18244"xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/title | "Molecular cloning of human plasma membrane phospholipid scramblase. A protein mediating transbilayer movement of plasma membrane phospholipids."xsd:string |
| http://purl.uniprot.org/citations/9218461 | http://purl.uniprot.org/core/title | "Molecular cloning of human plasma membrane phospholipid scramblase. A protein mediating transbilayer movement of plasma membrane phospholipids."xsd:string |