| http://purl.uniprot.org/citations/9219091 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9219091 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9219091 | http://www.w3.org/2000/01/rdf-schema#comment | "Cobalamin-dependent methionine synthase was purified from rat liver. The enzyme activity was separated into two peaks upon Mono-Q column chromatography. Peaks I and II of the enzyme, eluted in this order, were purified 18,000- and 44,000-fold in overall yields of 0.7 and 1.8%, respectively. Peak II methionine synthase, the major fraction, was homogeneous as judged by SDS-polyacrylamide gel electrophoresis. The enzyme was a large monomeric protein with an apparent molecular weight of 143,000 Da. Interconversion of the enzyme between the two peaks was not observed during purification procedures. The enzyme required S-adenosylmethionine and a reducing system for activity. Apparent K(m) values of the peak II enzyme for 5-methyltetrahydrofolate and homocysteine were 75 and 1.7 microM, respectively."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.org/dc/terms/identifier | "doi:10.3177/jnsv.43.177"xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.org/dc/terms/identifier | "doi:10.3177/jnsv.43.177"xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Yamada K."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Yamada K."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Wada M."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Wada M."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Kawata T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Kawata T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Maekawa A."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Maekawa A."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Tobimatsu T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Tobimatsu T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Toraya T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/author | "Toraya T."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/name | "J. Nutr. Sci. Vitaminol."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/name | "J. Nutr. Sci. Vitaminol."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/pages | "177-186"xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/pages | "177-186"xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/title | "Purification and some properties of cobalamin-dependent methionine synthase from rat liver."xsd:string |
| http://purl.uniprot.org/citations/9219091 | http://purl.uniprot.org/core/title | "Purification and some properties of cobalamin-dependent methionine synthase from rat liver."xsd:string |