| http://purl.uniprot.org/citations/9224719 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9224719 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9224719 | http://www.w3.org/2000/01/rdf-schema#comment | "Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists of four subunits of 160, 100, 73, and 30 kD. Here we report the isolation and characterization of a cDNA encoding the 30-kD polypeptide. Antibodies raised against this protein inhibit cleavage and polyadenylation and coimmunoprecipitate the other CPSF subunits. The protein sequence contains five C3H-zinc-finger repeats and a putative RNA-binding zinc knuckle motif at the carboxyl terminus. Consistent with this observation, the in vitro translated 30-kD protein binds RNA polymers with a distinct preference for poly(U). In addition, an essential S. cerevisiae gene, YTH1, was cloned which is 40% identical to CPSF 30K at the protein level. Extracts prepared from a conditional yth1 mutant have normal cleavage activity, but fail to polyadenylate the upstream cleavage product. Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.11.13.1703"xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.11.13.1703"xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Keller W."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Keller W."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Barabino S.M.L."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Barabino S.M.L."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Minvielle-Sebastia L."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Minvielle-Sebastia L."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Jenny A."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Jenny A."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Huebner W."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/author | "Huebner W."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/pages | "1703-1716"xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/pages | "1703-1716"xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/title | "The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/title | "The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins."xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/9224719 | http://purl.uniprot.org/core/volume | "11"xsd:string |