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http://purl.uniprot.org/citations/9224899http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9224899http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9224899http://www.w3.org/2000/01/rdf-schema#comment"We have isolated and sequenced a genomic clone for a pancreatic alpha-amylase gene (amy) of the chicken (Gallus gallus). The gene is interrupted by nine introns, spans over 4 kb, and encodes a protein (AMY) of 512 aa that is 83% identical to the human pancreatic alpha-amylase enzyme. Southern blot analysis of chicken DNA revealed two distinct pancreatic amy loci. In addition, we have generated a cDNA from chicken pancreatic RNA corresponding to the coding sequence of the genomic clone. The cDNA was inserted into a yeast expression vector, and the resulting construct used to transform Saccharomyces cerevisiae cells. Transformed yeast cells synthesized and secreted active AMY enzyme, and the gel migration pattern of the alpha-amylase produced by the yeast cells was identical to that of the native chicken enzyme."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.org/dc/terms/identifier"doi:10.1016/S0378-1119(97)00102-9"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.org/dc/terms/identifier"doi:10.1016/s0378-1119(97)00102-9"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Nguyen T."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Nguyen T."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Hickey D.A."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Hickey D.A."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Benkel B.F."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Benkel B.F."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Ahluwalia N."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Ahluwalia N."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Benkel K.I."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/author"Benkel K.I."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/name"Gene"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/pages"261-270"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/pages"261-270"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/title"Cloning and expression of a chicken alpha-amylase gene."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/title"Cloning and expression of a chicken alpha-amylase gene."xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/volume"192"xsd:string
http://purl.uniprot.org/citations/9224899http://purl.uniprot.org/core/volume"192"xsd:string