http://purl.uniprot.org/citations/9224899 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9224899 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9224899 | http://www.w3.org/2000/01/rdf-schema#comment | "We have isolated and sequenced a genomic clone for a pancreatic alpha-amylase gene (amy) of the chicken (Gallus gallus). The gene is interrupted by nine introns, spans over 4 kb, and encodes a protein (AMY) of 512 aa that is 83% identical to the human pancreatic alpha-amylase enzyme. Southern blot analysis of chicken DNA revealed two distinct pancreatic amy loci. In addition, we have generated a cDNA from chicken pancreatic RNA corresponding to the coding sequence of the genomic clone. The cDNA was inserted into a yeast expression vector, and the resulting construct used to transform Saccharomyces cerevisiae cells. Transformed yeast cells synthesized and secreted active AMY enzyme, and the gel migration pattern of the alpha-amylase produced by the yeast cells was identical to that of the native chicken enzyme."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.org/dc/terms/identifier | "doi:10.1016/S0378-1119(97)00102-9"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(97)00102-9"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Nguyen T."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Hickey D.A."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Hickey D.A."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Benkel B.F."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Benkel B.F."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Ahluwalia N."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Ahluwalia N."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Benkel K.I."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/author | "Benkel K.I."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/pages | "261-270"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/pages | "261-270"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/title | "Cloning and expression of a chicken alpha-amylase gene."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/title | "Cloning and expression of a chicken alpha-amylase gene."xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/volume | "192"xsd:string |
http://purl.uniprot.org/citations/9224899 | http://purl.uniprot.org/core/volume | "192"xsd:string |