| http://purl.uniprot.org/citations/9228013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9228013 | http://www.w3.org/2000/01/rdf-schema#comment | "Endothelial nitric-oxide synthase (eNOS) and caveolin-1 are associated within endothelial plasmalemmal caveolae. It is not known, however, whether eNOS and caveolin-1 interact directly or indirectly or whether the interaction affects eNOS activity. To answer these questions, we have cloned the bovine caveolin-1 cDNA and have investigated the eNOS-caveolin-1 interaction in an in vitro binding assay system using glutathione S-transferase (GST)-caveolin-1 fusion proteins and baculovirus-expressed bovine eNOS. We have also mapped the domains involved in the interaction using an in vivo yeast two-hybrid system. Results obtained using both in vitro and in vivo protein interaction assays show that both N- and C-terminal cytosolic domains of caveolin-1 interact directly with the eNOS oxygenase domain. Interaction of eNOS with GST-caveolin-1 fusion proteins significantly inhibits enzyme catalytic activity. A synthetic peptide corresponding to caveolin-1 residues 82-101 also potently and reversibly inhibits eNOS activity by interfering with the interaction of the enzyme with Ca2+/calmodulin (CaM). Regulation of eNOS in endothelial cells, therefore, may involve not only positive allosteric regulation by Ca2+/CaM, but also negative allosteric regulation by caveolin-1."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.30.18522"xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/author | "Ju H."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/author | "Venema V.J."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/author | "Venema R.C."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/author | "Zou R."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/pages | "18522-18525"xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/title | "Direct interaction of endothelial nitric-oxide synthase and caveolin-1 inhibits synthase activity."xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9228013 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9228013 |
| http://purl.uniprot.org/citations/9228013 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9228013 |
| http://purl.uniprot.org/uniprot/#_E9Q9X4-mappedCitation-9228013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9228013 |
| http://purl.uniprot.org/uniprot/#_P70313-mappedCitation-9228013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9228013 |
| http://purl.uniprot.org/uniprot/#_Q8C5P3-mappedCitation-9228013 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9228013 |
| http://purl.uniprot.org/uniprot/P70313 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9228013 |
| http://purl.uniprot.org/uniprot/Q8C5P3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9228013 |
| http://purl.uniprot.org/uniprot/E9Q9X4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9228013 |