| http://purl.uniprot.org/citations/9234759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9234759 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9234759 | http://www.w3.org/2000/01/rdf-schema#comment | "The Bacillus anthracis toxin genes, cya, lef, and pag, can be viewed as a regulon, in which transcription of all three genes is activated in trans by the same regulatory gene, atxA, in response to the same signal, CO2. In atxA+ strains, toxin gene expression is increased 5-to 20-fold in cells grown in 5% CO2 relative to cells grown in air. CO2-enhanced toxin gene transcription is not observed in atx4-null mutants. Here, we used two independent techniques to obtain evidence for additional CO2-induced atxA-regulated genes. First, total protein preparations from atxA4+ and atxA isolates grown in 5% CO2 and in air were examined by two-dimensional electrophoresis. Comparison of the resulting protein patterns indicated that synthesis of non-toxin proteins is influenced by growth in elevated CO2 and the toxin gene regulator, atxA. Second, we generated random transcriptional lacZ fusions in B. anthracis with transposon Tn917-LTV3. Transposon-insertion libraries were screened for mutants expressing CO2-enhanced atxA-dependent beta-galactosidase activity. DNA sequence analysis of transposon insertion sites in 17 mutants carrying CO2- and atxA-regulated fusions revealed 10 mutants carrying independent insertions on the 185-kb toxin plasmid pXO1 which did not map to the toxin genes. The tcr-lacZ fusion mutants (tcr for toxin coregulated) were Tox+, indicating that these genes may not be involved in anthrax toxin gene activation. Our data indicate a clear association of atxA with CO2-enhanced gene expression in B. anthracis and provide evidence that atxA regulates genes other than the structural genes for the anthrax toxin proteins."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.65.8.3091-3099.1997"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.65.8.3091-3099.1997"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/author | "Koehler T.M."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/author | "Koehler T.M."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/author | "Hoffmaster A.R."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/author | "Hoffmaster A.R."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/pages | "3091-3099"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/pages | "3091-3099"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/title | "The anthrax toxin activator gene atxA is associated with CO2-enhanced non-toxin gene expression in Bacillus anthracis."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/title | "The anthrax toxin activator gene atxA is associated with CO2-enhanced non-toxin gene expression in Bacillus anthracis."xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://purl.uniprot.org/core/volume | "65"xsd:string |
| http://purl.uniprot.org/citations/9234759 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9234759 |
| http://purl.uniprot.org/citations/9234759 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9234759 |
| http://purl.uniprot.org/citations/9234759 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9234759 |
| http://purl.uniprot.org/citations/9234759 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9234759 |
| http://purl.uniprot.org/uniprot/Q44636 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9234759 |
| http://purl.uniprot.org/uniprot/O07491 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9234759 |