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| http://purl.uniprot.org/citations/9235896 | http://www.w3.org/2000/01/rdf-schema#comment | "The alpha-subunit of eukaryotic initiation factor 2B (eIF-2B), a guanine nucleotide exchange protein that functions in regulation of translation, was observed to associate with the carboxyl-terminal cytoplasmic domains of the alpha2A- and alpha2B-adrenergic receptors in a yeast two-hybrid screen of a cDNA library prepared from 293 cells. This protein association was confirmed in vitro by affinity chromatography and was shown to be specific for a subset of G protein-coupled receptors, including the alpha2A-, alpha2B-, alpha2C-, and beta2-adrenergic receptors, but not the vasopressin (V2) receptor. Association of these proteins in vivo was confirmed by specific co-immunoprecipitation of eIF-2Balpha with full-length beta2-adrenergic receptors expressed in transfected 293 cells and by fluorescence microscopy showing co-localization of these proteins in intact cells. Remarkably, eIF-2Balpha co-localized with receptors exclusively in regions of the plasma membrane that are in contact with the extracellular medium, but failed to associate with membranes making cell-cell contacts. Overexpression of eIF-2Balpha in 293 cells caused a small (approximately 15%) but significant enhancement of beta2-adrenergic receptor-mediated activation of adenylyl cyclase, without affecting forskolin or V2 receptor-mediated activation. These observations suggest a new role for a previously identified guanine nucleotide exchange protein in membrane biology and cell signaling."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.31.19099"xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/author | "Kobilka B.K."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/author | "Klein U."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/author | "von Zastrow M."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/author | "Ramirez M.T."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/pages | "19099-19102"xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/title | "A novel interaction between adrenergic receptors and the alpha-subunit of eukaryotic initiation factor 2B."xsd:string |
| http://purl.uniprot.org/citations/9235896 | http://purl.uniprot.org/core/volume | "272"xsd:string |
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