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http://purl.uniprot.org/citations/9244264http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9244264http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9244264http://www.w3.org/2000/01/rdf-schema#comment"An 8.5-kb plasmid encoding genes (leuABCD) involved in leucine biosynthesis and a small plasmid of 1.74 kb of yet unknown function were found in the intracellular symbiont, Buchnera aphidicola, of two divergent aphid species, Thelaxes suberi and Tetraneura caerulescens, respectively. The leuABCD-carrying plasmid (pBTs1) was amplified from total aphid DNA by inverse long PCR, using outwardly oriented oligonucleotide primers specific to leuA. The resulting 8.2-kb PCR fragment as well as the 1.74-kb plasmid (pBTc1) were cloned and sequenced. pBTs1 differed from a previously described B. aphidicola plasmid (pRPE) of the aphid Rhopalosiphum padi by the presence of a small heat shock gene (ibp) and in the order of the leuABCD and repA genes. Comparison of both leucine plasmids to the small plasmid pBTc1 revealed extensive similarity with respect to putative replication functions as well as in the presence of a highly conserved open reading frame that was found to be homologous to Escherichia coli YqhA and Haemophilus influenzae HI0507 and which may encode an integral membrane protein. The three B. aphidicola plasmids most likely evolved from a common ancestral replicon, which in turn may be distantly related to IncFII plasmids. Phylogenetic affiliations of the B. aphidicola strains of the two aphid species were assessed by sequencing of their 16S rRNA genes. Evaluation of the distribution of the leuABCD-encoding plasmids within a phylogenetic framework suggests independent origins for pBTs1 and pRPE from an ancestral replicon resembling pBTc1. The implications for symbiotic essential amino acid biosynthesis and provisioning are discussed."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.org/dc/terms/identifier"doi:10.1128/jb.179.15.4768-4777.1997"xsd:string
http://purl.uniprot.org/citations/9244264http://purl.org/dc/terms/identifier"doi:10.1128/jb.179.15.4768-4777.1997"xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"Latorre A."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"Latorre A."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"Moya A."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"Moya A."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"van Ham R.C.H.J."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"van Ham R.C.H.J."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/author"Van Ham R.C.H.J."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/pages"4768-4777"xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/pages"4768-4777"xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/title"Putative evolutionary origin of plasmids carrying the genes involved in leucine biosynthesis in Buchnera aphidicola (endosymbiont of aphids)."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/title"Putative evolutionary origin of plasmids carrying the genes involved in leucine biosynthesis in Buchnera aphidicola (endosymbiont of aphids)."xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/volume"179"xsd:string
http://purl.uniprot.org/citations/9244264http://purl.uniprot.org/core/volume"179"xsd:string
http://purl.uniprot.org/citations/9244264http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9244264
http://purl.uniprot.org/citations/9244264http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9244264
http://purl.uniprot.org/citations/9244264http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/9244264