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http://purl.uniprot.org/citations/9263451http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9263451http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9263451http://www.w3.org/2000/01/rdf-schema#comment"Three types of Arabidopsis cDNA (cANP1, cANP2 and cANP3) have been isolated that encode putative protein kinases, designated ANP1, ANP2 and ANP3. These kinases exhibit a high degree of homology to NPK1, a tobacco protein that is a member of the family of mitogen-activated protein kinase kinase kinases (MAPKKKs), which appears to function in the proliferation of tobacco cells. The predicted amino acid sequences of the kinase domains in the amino-terminal halves of the ANPs were more than 80% identical to that of NPK1, while the kinase-unrelated regions in the carboxy-terminal halves exhibited relatively low homology. Two species of cANP1 were identified, ANP1L cDNA (cANP1L) and ANP1S cDNA (cANP1S), which were derived from a single ANP1 gene: the former had an intron-like sequence in the coding region for the kinase-unrelated region, while the latter did not include such an intron-like sequence. cANP1L encoded a putative protein with both kinase and kinase-unrelated domains, resembling NPK1, whereas cANP1S encoded only the amino-terminal kinase domain because the intron-like sequence was absent, with resulting elimination of most of the kinase-unrelated region. Genetic analysis with mutant yeast cells showed that over-expression of cANP1L or of cANP1S activated the mating pheromone-responsive signal pathway which is mediated by a MAP kinase cascade. Moreover, the extent of such activation by cANP1S was greater than that by cANP1L. These results predict that differential splicing of the intron-like sequence in the ANP1 transcript might be at least one of the molecular mechanisms involved in the generation of active ANP1 protein kinase."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-313x.1997.12010039.x"xsd:string
http://purl.uniprot.org/citations/9263451http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-313x.1997.12010039.x"xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Irie K."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Irie K."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Machida Y."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Machida Y."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Banno H."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Banno H."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Kawahara E."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Kawahara E."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Nishihama R."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/author"Nishihama R."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/name"Plant J."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/name"Plant J."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/pages"39-48"xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/pages"39-48"xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/title"Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/title"Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)."xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/9263451http://purl.uniprot.org/core/volume"12"xsd:string