| http://purl.uniprot.org/citations/9293008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9293008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9293008 | http://www.w3.org/2000/01/rdf-schema#comment | "The gene encoding the hyperthermophilic extracellular alpha-amylase from Pyrococcus furiosus was cloned by activity screening in Escherichia coli. The gene encoded a single 460-residue polypeptide chain. The polypeptide contained a 26-residue signal peptide, indicating that this Pyrococcus alpha-amylase was an extracellular enzyme. Unlike the P. furiosus intracellular alpha-amylase, this extracellular enzyme showed 45 to 56% similarity and 20 to 35% identity to other amylolytic enzymes of the alpha-amylase family and contained the four consensus regions characteristic of that enzyme family. The recombinant protein was a homodimer with a molecular weight of 100,000, as estimated by gel filtration. Both the dimer and monomer retained starch-degrading activity after extensive denaturation and migration on sodium dodecyl sulfate-polyacrylamide gels. The P. furiosus alpha-amylase was a liquefying enzyme with a specific activity of 3,900 U mg-1 at 98 degrees C. It was optimally active at 100 degrees C and pH 5.5 to 6.0 and did not require Ca2+ for activity or thermostability. With a half-life of 13 h at 98 degrees C, the P. furiosus enzyme was significantly more thermostable than the commercially available Bacillus licheniformis alpha-amylase (Taka-therm)."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.63.9.3569-3576.1997"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Dong G."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Dong G."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Savchenko A."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Savchenko A."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Vieille C."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Vieille C."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Zeikus J.G."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/author | "Zeikus J.G."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/name | "Appl Environ Microbiol"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/pages | "3569-3576"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/pages | "3569-3576"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/title | "Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/title | "Cloning, sequencing, and expression of the gene encoding extracellular alpha-amylase from Pyrococcus furiosus and biochemical characterization of the recombinant enzyme."xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/volume | "63"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://purl.uniprot.org/core/volume | "63"xsd:string |
| http://purl.uniprot.org/citations/9293008 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9293008 |
| http://purl.uniprot.org/citations/9293008 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9293008 |
| http://purl.uniprot.org/citations/9293008 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9293008 |