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http://purl.uniprot.org/citations/9298645http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9298645http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9298645http://www.w3.org/2000/01/rdf-schema#comment"Following the complete sequencing of the genome of the univellular cyanobacterium, Synechocystis sp. strain PCC6803 within our institute, a protein-gene linkage map of this photosynthetic microorganism was successfully constructed for 130 high abundance proteins present on two-dimensional gels. An additional six proteins were analyzed, but were probably encoded extrachromosomally. In order to demonstrate the usefulness of this protein-gene linkage map, we analyzed the changes that occur in cellular proteins after illumination of PCC6803 cells. The results indicate that this protein-gene linkage map greatly simplifies the identification process of such modulated genes. After illumination, at least three distinctive spots with reduced intensity were detected on two-dimensional gels and the corresponding genes of two of these were successfully identified as chaperonin 2 and a Tortula ruralis rehydrin-related gene. Thus, the combination of the protein-gene linkage map and two-dimensional gel electrophoresis should permit a comprehensive analyses of the proteins encoded by the genome (i.e., "proteome") of this photosynthetic autotroph. This post-genome project represents a productive way of exploiting the information obtained from the sequencing of the cyanobacterium genome."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.org/dc/terms/identifier"doi:10.1002/elps.1150180806"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.org/dc/terms/identifier"doi:10.1002/elps.1150180806"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/author"Ohara O."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/author"Ohara O."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/author"Sazuka T."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/author"Sazuka T."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/name"Electrophoresis"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/name"Electrophoresis"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/pages"1252-1258"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/pages"1252-1258"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/title"Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: linking 130 protein spots with their respective genes."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/title"Towards a proteome project of cyanobacterium Synechocystis sp. strain PCC6803: linking 130 protein spots with their respective genes."xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/9298645http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/9298645http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9298645
http://purl.uniprot.org/citations/9298645http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9298645
http://purl.uniprot.org/citations/9298645http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/9298645
http://purl.uniprot.org/citations/9298645http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/9298645
http://purl.uniprot.org/uniprot/P26290http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/9298645
http://purl.uniprot.org/uniprot/P74070http://purl.uniprot.org/core/citationhttp://purl.uniprot.org/citations/9298645