| http://purl.uniprot.org/citations/9305631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9305631 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9305631 | http://www.w3.org/2000/01/rdf-schema#comment | "The 70 kDa heat shock family of molecular chaperones is essential to a variety of cellular processes, yet it is unclear how these proteins are regulated in vivo. We present evidence that the protein BAG-1 is a potential modulator of the molecular chaperones, Hsp70 and Hsc70. BAG-1 binds to the ATPase domain of Hsp70 and Hsc70, without requirement for their carboxy-terminal peptide-binding domain, and can be co-immunoprecipitated with Hsp/Hsc70 from cell lysates. Purified BAG-1 and Hsp/Hsc70 efficiently form heteromeric complexes in vitro. BAG-1 inhibits Hsp/Hsc70-mediated in vitro refolding of an unfolded protein substrate, whereas BAG-1 mutants that fail to bind Hsp/Hsc70 do not affect chaperone activity. The binding of BAG-1 to one of its known cellular targets, Bcl-2, in cell lysates was found to be dependent on ATP, consistent with the possible involvement of Hsp/Hsc70 in complex formation. Overexpression of BAG-1 also protected certain cell lines from heat shock-induced cell death. The identification of Hsp/Hsc70 as a partner protein for BAG-1 may explain the diverse interactions observed between BAG-1 and several other proteins, including Raf-1, steroid hormone receptors and certain tyrosine kinase growth factor receptors. The inhibitory effects of BAG-1 on Hsp/Hsc70 chaperone activity suggest that BAG-1 represents a novel type of chaperone regulatory proteins and thus suggest a link between cell signaling, cell death and the stress response."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.16.4887"xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.16.4887"xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Xie Z."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Xie Z."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Reed J.C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Reed J.C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Takayama S."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Takayama S."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Aime-Sempe C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Aime-Sempe C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Bimston D.N."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Bimston D.N."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Freeman B.C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Freeman B.C."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Matsuzawa S.-I."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Matsuzawa S.-I."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Morimoto R.I."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/author | "Morimoto R.I."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9305631 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |