| http://purl.uniprot.org/citations/9311131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9311131 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9311131 | http://www.w3.org/2000/01/rdf-schema#comment | "The nucleotide sequence of the bphB gene of Pseudomonas putida strain OU83 was determined. The bphB gene, which encodes cis-biphenyl dihydrodiol dehydrogenase (BDDH), was composed of 834 base pairs with an ATG initiation codon and a TGA termination codon. It can encode a polypeptide of 28.91 kDa, containing 277 amino acids. Promoter-like and ribosome-binding sequences were identified upstream of the bphB gene. The bphB nucleotide sequence was used to produce His-tagged BDDH, in Escherichia coli. The His-tagged BDDH construction, carrying a single 6 x His tail on the N-terminal portion, was active. The molecular mass of the native enzyme was 128 kDa and on SDS-PAGE analysis the molecular mass was 31 kDa. This enzyme requires NAD+ for its activity and its optimum pH is 8.5. Nucleotide and the deduced amino acid sequence analyses revealed a high degree of homology between the bphB gene from Pseudomonas putida OU83 and the bphB genes from P. cepacia LB400 and P. pseudoalcaligenes KF707."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.1997.tb12662.x"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1574-6968.1997.tb12662.x"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Cerniglia C.E."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Cerniglia C.E."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Khan A.A."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Khan A.A."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Wang R.F."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Wang R.F."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Nawaz M.S."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/author | "Nawaz M.S."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/name | "FEMS Microbiol. Lett."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/pages | "317-324"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/pages | "317-324"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/title | "Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol dehydrogenase (bphB) and the expression of an active recombinant His-tagged bphB gene product from a PCB degrading bacterium, Pseudomonas putida OU83."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/title | "Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol dehydrogenase (bphB) and the expression of an active recombinant His-tagged bphB gene product from a PCB degrading bacterium, Pseudomonas putida OU83."xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/volume | "154"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://purl.uniprot.org/core/volume | "154"xsd:string |
| http://purl.uniprot.org/citations/9311131 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9311131 |
| http://purl.uniprot.org/citations/9311131 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9311131 |