| http://purl.uniprot.org/citations/9341124 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9341124 | http://www.w3.org/2000/01/rdf-schema#comment | "Neurocan is a member of the aggrecan family of proteoglycans which are characterized by NH2-terminal domains binding hyaluronan, and COOH-terminal domains containing C-type lectin-like modules. To detect and enhance the affinity for complementary ligands of neurocan, the COOH-terminal neurocan domain was fused with the NH2-terminal region of tenascin-C, which contains the hexamerization domain of this extracellular matrix glycoprotein. The fusion protein was designed to contain the last downstream glycosaminoglycan attachment site and was expressed as a proteoglycan. In ligand overlay blots carried out with brain extracts, it recognized tenascin-C. The interaction was abolished by the addition of EDTA, or TNfn4,5, a bacterially expressed tenascin-C fragment comprising the fourth and fifth fibronectin type III module. The fusion protein directly reacted with this fragment in ligand blot and enzyme-linked immunosorbent assay procedures. Both tenascin-C and TNfn4,5 were retained on Sepharose 4B-linked carboxyl-terminal neurocan domains, which in BIAcore binding studies yielded a KD value of 17 nM for purified tenascin-C. We conclude that a divalent cation-dependent interaction between the COOH-terminal domain of neurocan and those fibronectin type III repeats is substantially involved in the binding of neurocan to tenascin-C."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.43.26905"xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Fassler R."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Clement A."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Rauch U."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Gohring W."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Faissner A."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Retzler C."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/author | "Frohlich L."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/pages | "26905-26912"xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/title | "Mapping of a defined neurocan binding site to distinct domains of tenascin-C."xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://purl.uniprot.org/core/volume | "272"xsd:string |
| http://purl.uniprot.org/citations/9341124 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9341124 |
| http://purl.uniprot.org/citations/9341124 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9341124 |
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| http://purl.uniprot.org/uniprot/#_Q9UQP3-mappedCitation-9341124 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9341124 |