| http://purl.uniprot.org/citations/9346907 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9346907 | http://www.w3.org/2000/01/rdf-schema#comment | "Wortmannin is a natural product that inhibits signal transduction. One target of wortmannin in mammalian cells is the 110-kDa catalytic subunit of phosphatidylinositol 3-kinase (PI 3-kinase). We show that wortmannin is toxic to the yeast Saccharomyces cerevisiae and present genetic and biochemical evidence that a phosphatidylinositol 4-kinase (PI 4-kinase), STT4, is a target of wortmannin in yeast. In a strain background in which stt4 mutants are rescued by osmotic support with sorbitol, the toxic effects of wortmannin are similarly prevented by sorbitol. In contrast, in a different strain background, STT4 is essential under all conditions and wortmannin toxicity is not mitigated by sorbitol. Overexpression of STT4 confers wortmannin resistance, but overexpression of PIK1, a related PI 4-kinase, does not. In vitro, the PI 4-kinase activity of STT4, but not of PIK1, was potently inhibited by wortmannin. Overexpression of the phosphatidylinositol 4-phosphate 5-kinase homolog MSS4 conferred wortmannin resistance, as did deletion of phospholipase C-1. These observations support a model for a phosphatidylinositol metabolic cascade involving STT4, MSS4, and phospholipase C-1 and provide evidence that an essential product of this pathway is the lipid phosphatidylinositol 4,5-bisphosphate."xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.44.27671"xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/author | "Heitman J."xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/author | "Cardenas M.E."xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/author | "Cutler N.S."xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/pages | "27671-27677"xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/title | "STT4 is an essential phosphatidylinositol 4-kinase that is a target of wortmannin in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/9346907 | http://purl.uniprot.org/core/volume | "272"xsd:string |
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