| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/2000/01/rdf-schema#comment | "The enzyme which seems to be responsible for the formation of the enantiomeric configuration of the glycerophosphate backbone (sn-glycerol-1-phosphate) of archaeal ether lipids was purified from a methanogenic archaeon, Methanobacterium thermoautotrophicum, and characterized. The enzyme, sn-glycerol-1-phosphate: NAD(P)+ oxidoreductase (sn-glycerol-1-phosphate dehydrogenase), was purified 7,600-fold from a cell free extract by ammonium sulfate fractionation and seven steps of chromatography. The final preparation exhibited a specific activity of 617 micromol/min/mg (Vmax) and gave a single band corresponding to 38 kDa on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The native enzyme showed an apparent molecular mass of 302 kDa on gel-filtration chromatography, indicating it is present as a homooctamer. Maximum activity was observed at 75 degrees C at near neutral pH. The activity was stimulated by potassium ions. The Km for dihydroxyacetone phosphate was 7.5 times smaller than that for sn-glycerol-1-phosphate, suggesting that the formation of sn-glycerol-1-phosphate is the natural direction in the cell. Under the assay conditions used, no product inhibition was observed. The N-terminal amino acid sequence was determined."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a021791"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a021791"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/author | "Nishihara M."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/author | "Nishihara M."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/author | "Koga Y."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/author | "Koga Y."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/pages | "572-576"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/pages | "572-576"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/title | "Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/title | "Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea."xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/volume | "122"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://purl.uniprot.org/core/volume | "122"xsd:string |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9348086 |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9348086 |
| http://purl.uniprot.org/citations/9348086 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9348086 |
| http://purl.uniprot.org/citations/9348086 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9348086 |
| http://purl.uniprot.org/citations/9348086 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9348086 |