| http://purl.uniprot.org/citations/9353262 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9353262 | http://www.w3.org/2000/01/rdf-schema#comment | "Shortly after initiating promoter-specific transcription in vitro, mammalian RNA polymerase II becomes highly susceptible to arrest in a promoter-proximal region 9-13 base pairs downstream of the transcriptional start site (Dvir, A., Conaway, R. C., and Conaway, J. W. (1996) J. Biol. Chem. 271, 23352-23356). Arrest by polymerase in this region is suppressed by TFIIH in an ATP-dependent reaction (Dvir, A., Conaway, R. C., and Conaway, J. W. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 9006-9010). In this report, we present evidence that, in addition to TFIIH and an ATP cofactor, efficient transcription by RNA polymerase II through this promoter-proximal region requires formation of an "escape-competent" transcriptional intermediate. Formation of this intermediate requires template DNA 40-50 base pairs downstream of the transcriptional start site. This requirement for downstream DNA is transient, since template DNA downstream of +40 is dispensable for assembly of the preinitiation complex, for initiation and synthesis of the first 10-12 phosphodiester bonds of nascent transcripts and for further extension of transcripts longer than approximately 14 nucleotides. Thus, promoter escape requires that the RNA polymerase II transcription complex undergoes a critical structural transition, likely driven by interaction of one or more components of the transcriptional machinery with template DNA 40-50 base pairs downstream of the transcriptional start site."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.272.45.28175"xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/author | "Tan S."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/author | "Conaway J.W."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/author | "Conaway R.C."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/author | "Dvir A."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/pages | "28175-28178"xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/title | "Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter."xsd:string |
| http://purl.uniprot.org/citations/9353262 | http://purl.uniprot.org/core/volume | "272"xsd:string |
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