| http://purl.uniprot.org/citations/9361024 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9361024 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9361024 | http://www.w3.org/2000/01/rdf-schema#comment | "Huntington's disease (HD) is an inherited neurodegenerative disease caused by expansion of a polyglutamine repeat in the HD protein huntingtin. Huntingtin's localization within the cell includes an association with cytoskeletal elements and vesicles. We previously identified a protein (HAP1) which binds to huntingtin in a glutamine repeat length-dependent manner. We now report that HAP1 interacts with cytoskeletal proteins, namely the p150 Glued subunit of dynactin and the pericentriolar protein PCM-1. Structural predictions indicate that both HAP1 and the interacting proteins have a high probability of forming coiled coils. We examined the interaction of HAP1 with p150 Glued . Binding of HAP1 to p150 Glued (amino acids 879-1150) was confirmed in vitro by binding of p150 Glued to a HAP1-GST fusion protein immobilized on glutathione-Sepharose beads. Also, HAP1 co-immunoprecipitated with p150 Glued from brain extracts, indicating that the interaction occurs in vivo . Like HAP1, p150 Glued is highly expressed in neurons in brain and both proteins are enriched in a nerve terminal vesicle-rich fraction. Double label immunofluorescence experiments in NGF-treated PC12 cells using confocal microscopy revealed that HAP1 and p150 Glued partially co-localize. These results suggest that HAP1 might function as an adaptor protein using coiled coils to mediate interactions among cytoskeletal, vesicular and motor proteins. Thus, HAP1 and huntingtin may play a role in vesicle trafficking within the cell and disruption of this function could contribute to the neuronal dysfunction and death seen in HD."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/6.13.2205"xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.org/dc/terms/identifier | "doi:10.1093/hmg/6.13.2205"xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Ross C.A."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Ross C.A."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Sharp A.H."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Sharp A.H."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Engelender S."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Engelender S."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Tokito M.K."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Tokito M.K."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Holzbaur E.L.F."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Holzbaur E.L.F."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Lanahan A."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Lanahan A."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Colomer V."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Colomer V."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Worley P."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/author | "Worley P."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |
| http://purl.uniprot.org/citations/9361024 | http://purl.uniprot.org/core/name | "Hum. Mol. Genet."xsd:string |