| http://purl.uniprot.org/citations/9361436 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9361436 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9361436 | http://www.w3.org/2000/01/rdf-schema#comment | "The DNA polymerase gene from the archaeon Pyrococcus sp. strain KOD1 (KOD DNA polymerase) contains a long open reading frame of 5,013 bases that encodes 1,671 amino acid residues (GenBank accession no. D29671). Similarity analysis revealed that the DNA polymerase contained a putative 3'-5' exonuclease activity and two in-frame intervening sequences of 1,080 bp (360 amino acids; KOD pol intein-1) and 1,611 bp (537 amino acids; KOD pol intein-2), which are located in the middle of regions conserved among eukaryotic and archaeal alpha-like DNA polymerases. The mature form of the DNA polymerase gene was expressed in Escherichia coli, and the recombinant enzyme was purified and characterized. 3'-5' exonuclease activity was confirmed, and although KOD DNA polymerase's optimum temperature (75 degrees C) and mutation frequency (3.5 x 10(-3)) were similar to those of a DNA polymerase from Pyrococcus furiosus (Pfu DNA polymerase), the KOD DNA polymerase exhibited an extension rate (100 to 130 nucleotides/s) 5 times higher and a processivity (persistence of sequential nucleotide polymerization) 10 to 15 times higher than those of Pfu DNA polymerase. These characteristics enabled the KOD DNA polymerase to perform a more accurate PCR in a shorter reaction time."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.63.11.4504-4510.1997"xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.org/dc/terms/identifier | "doi:10.1128/aem.63.11.4504-4510.1997"xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Inoue H."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Inoue H."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Imanaka T."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Imanaka T."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kawakami B."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kawakami B."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Oka M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Oka M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kakihara H."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kakihara H."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kitabayashi M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Kitabayashi M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Nishioka M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/author | "Nishioka M."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/9361436 | http://purl.uniprot.org/core/name | "Appl. Environ. Microbiol."xsd:string |