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http://purl.uniprot.org/citations/9362117http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9362117http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9362117http://www.w3.org/2000/01/rdf-schema#comment"To obtain genes with sequence similarity to aminopeptidase T (AP-T) of Thermus aquaticus YT-1, we cloned the genes encoding aminopeptidase Th (AP-Th) from Thermus thermophilus HB8 and aminopeptidase II (APII) from Bacillus stearothermophilus NCIB8924. The AP-Th gene encoded a polypeptide of 408 amino acid residues and the deduced molecular weight of this subunit was 45,015. The APII gene encoded a polypeptide of 413 amino acid residues with a deduced molecular weight of 46,207. The extent of amino acid sequence similarity between AP-Th and AP-T was 86%, and that between APII and AP-T was 43%. The substrate specificities of these expressed enzymes were similar, and each efficiently hydrolyzed leucyl- or phenyl-peptide substrates. Since the deduced amino acid sequence of these enzymes show no similarity to other known aminopeptidases, they appear to comprise an independent family of peptidases, designated the AP-T family. However, a conserved region within the enzymes of the AP-T family shows similarity to the active site signature of the leucyl aminopeptidase family, suggesting that these enzymes may belong to the leucyl aminopeptidase superfamily."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.org/dc/terms/identifier"doi:10.1271/bbb.61.1710"xsd:string
http://purl.uniprot.org/citations/9362117http://purl.org/dc/terms/identifier"doi:10.1271/bbb.61.1710"xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Kaminogawa S."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Kaminogawa S."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Minagawa E."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Minagawa E."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Motoshima H."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Motoshima H."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Tsukasaki F."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/author"Tsukasaki F."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/name"Biosci. Biotechnol. Biochem."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/name"Biosci. Biotechnol. Biochem."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/pages"1710-1717"xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/pages"1710-1717"xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/title"Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/title"Cloning of genes of the aminopeptidase T family from Thermus thermophilus HB8 and Bacillus stearothermophilus NCIB8924: apparent similarity to the leucyl aminopeptidase family."xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/volume"61"xsd:string
http://purl.uniprot.org/citations/9362117http://purl.uniprot.org/core/volume"61"xsd:string
http://purl.uniprot.org/citations/9362117http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9362117
http://purl.uniprot.org/citations/9362117http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9362117