| http://purl.uniprot.org/citations/9362500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9362500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9362500 | http://www.w3.org/2000/01/rdf-schema#comment | "Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA-dependent protein kinase (DNA-PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA-PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double-strand breaks, and V(D)J recombination product formation defects. Here we show that the IR repair, DNA end binding and DNA-PK defects in Ku70-/-embryonic stem cells can be counteracted by introducing epitope-tagged wild-type Ku70 cDNA. Truncations and chimeras of Ku70 were used to identify the regions necessary for DNA end binding and IR repair. Site-specific mutational analysis revealed a core region of Ku70 responsible for DNA end binding and heterodimerization. The propensity for Ku70 to associate with Ku80 and to bind DNA correlates with the ability to activate DNA-PK, although two mutants showed that the roles of Ku70 in DNA-PK activation and IR repair are separate. Mutation of DNA-PK autophosphorylation sites and other structural motifs in Ku70 showed that these sites are not necessary for IR repair in vivo. These studies reveal Ku70 features required for double-strand break repair."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.22.6874"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/16.22.6874"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/author | "Jin S."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/author | "Jin S."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/author | "Weaver D.T."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/author | "Weaver D.T."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/pages | "6874-6885"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/pages | "6874-6885"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/title | "Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/title | "Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions."xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/9362500 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9362500 |
| http://purl.uniprot.org/citations/9362500 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9362500 |
| http://purl.uniprot.org/citations/9362500 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9362500 |
| http://purl.uniprot.org/citations/9362500 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9362500 |
| http://purl.uniprot.org/uniprot/P12956 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9362500 |
| http://purl.uniprot.org/uniprot/P78527 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9362500 |