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http://purl.uniprot.org/citations/9371457http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9371457http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9371457http://www.w3.org/2000/01/rdf-schema#comment"Bacteria employ autoinduction systems to sense the onset of appropriate cell density for expression of developmental genes. In many gram-negative bacteria, autoinduction involves the production of and response to diffusible acylated-homoserine lactones (acyl-HSLs) and is mediated by members of the LuxR and LuxI families. Ralstonia (Pseudomonas) solanacearum, a phytopathogenic bacterium that appears to autoregulate its virulence genes, produces compounds that promote expression of several heterologous acyl-HSL-responsive reporter gene constructs. High-pressure liquid chromatography of highly concentrated ethyl acetate extracts revealed that culture supernatants of strain AW1 contained two compounds with retention times similar to N-hexanoyl- and N-octanoyl-HSL. To investigate the role of these acyl-HSLs in R. solanacearum virulence gene expression, transposon mutants that were deficient for inducing an acyl-HSL-responsive reporter in Agrobacterium tumefaciens were generated. Three loci involved in normal acyl-HSL production were identified, one of which was shown to contain the divergently transcribed solR and solI genes, the luxR and luxI homologs, respectively. A 4.1-kb fragment containing solR and solI enabled all of the mutants (regardless of the locus inactivated) and a naturally acyl-HSL-defective strain of R. solanacearum to produce acyl-HSLs. Inactivation of solI abolished production of all detectable acyl-HSLs but affected neither the expression of virulence genes in culture nor the ability to wilt tomato plants. AW1 has a functional autoinduction system, because (i) expression of solI required SolR and acyl-HSL and (ii) expression of a gene linked to solR and solI, designated aidA, was acyl-HSL dependent. Because AidA has no homologs in the protein databases, its discovery provided no clues as to the role of acyl-HSLs in R. solanacearum gene regulation. However, expression of solR and solI required the global LysR-type virulence regulator PhcA, and both solR and solI exhibited a cell density-associated pattern of expression similar to other PhcA-regulated genes. The acyl-HSL-dependent autoinduction system in R. solanacearum is part of a more complex autoregulatory hierarchy, since the transcriptional activity of PhcA is itself controlled by a novel autoregulatory system that responds to 3-hydroxypalmitic acid methyl ester."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.org/dc/terms/identifier"doi:10.1128/jb.179.22.7089-7097.1997"xsd:string
http://purl.uniprot.org/citations/9371457http://purl.org/dc/terms/identifier"doi:10.1128/jb.179.22.7089-7097.1997"xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Schell M.A."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Schell M.A."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Denny T.P."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Denny T.P."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Flavier A.B."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Flavier A.B."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Ganova-Raeva L.M."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/author"Ganova-Raeva L.M."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/date"1997"xsd:gYear
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/name"J. Bacteriol."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/pages"7089-7097"xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/pages"7089-7097"xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/title"Hierarchical autoinduction in Ralstonia solanacearum: control of acyl-homoserine lactone production by a novel autoregulatory system responsive to 3-hydroxypalmitic acid methyl ester."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/title"Hierarchical autoinduction in Ralstonia solanacearum: control of acyl-homoserine lactone production by a novel autoregulatory system responsive to 3-hydroxypalmitic acid methyl ester."xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/volume"179"xsd:string
http://purl.uniprot.org/citations/9371457http://purl.uniprot.org/core/volume"179"xsd:string
http://purl.uniprot.org/citations/9371457http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9371457
http://purl.uniprot.org/citations/9371457http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9371457
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