| http://purl.uniprot.org/citations/9387880 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9387880 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9387880 | http://www.w3.org/2000/01/rdf-schema#comment | "Cells immunoreactive to antisera specifically directed against Lymnaea stagnalis caudo dorsal cells egg-laying hormone (CDCH) or against alpha- and beta-peptides (CDCP), encoded on the egg-laying hormone precursor, were detected in central nervous system (CNS) of the rhynchobdellid leech Theromyzon tessulatum. A co-localization of the CDC-like hormone and CDC-like peptides was found in T. tessulatum as in L. stagnalis CNS. approximately 45 immunoreactive cells to the anti-CDCH were detected in leech brain but this number varies according to the stage of the animal life cycle, i.e. it reaches a maximum just before egg-laying while after it decreases to 2-3 cells. CDCH and alpha-CDCP epitopes recognized by anti-CDCH and anti-alpha-CDCP were contained in neurosecretory granules. Following an extensive purification, including HPGPC and reverse-phase HPLC, the CDC-like hormone contained in the T. tessulatum CNA was isolated. The sequence (GSGVSNGGTEMIQLSHIRERQRYWAQDNLRRRFLEK-amide) was established by a combination of automated Edman degradation, arginyl-endopeptidase digestion, electrospray mass spectrometry measurement and carboxypeptidase A treatment. The results demonstrate that the peptide recognized by the anti-CDCH in the leech CNS possesses 27.8, 37.2 and 47.2% sequence identity with Aplysia parvula, Lymnaea stagnalis and Aplysia californica ELH, respectively. This molecule was named the leech egg-laying-like hormone (L-ELH). The secondary structure prediction of the L-ELH and all mollusks ELH, revealed the existence of a conserved segment (segment 29-34) in a strong helicoidal bend that might be important for receptor recognition and/or activation. This finding constitutes the first biochemical characterization of an egg-laying hormone in other invertebrates than mollusks."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/name | "Brain Res. Mol. Brain Res."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/name | "Brain Res. Mol. Brain Res."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0169-328x(97)00145-9"xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0169-328x(97)00145-9"xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9387880 |
| http://purl.uniprot.org/citations/9387880 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9387880 |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Salzet M."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Salzet M."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Vandenbulcke F."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Vandenbulcke F."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "van Minnen J."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "van Minnen J."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Verger-Bocquet M."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/author | "Verger-Bocquet M."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/pages | "211-221"xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/pages | "211-221"xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/title | "Leech egg-laying-like hormone: structure, neuronal distribution and phylogeny."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/title | "Leech egg-laying-like hormone: structure, neuronal distribution and phylogeny."xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/volume | "49"xsd:string |
| http://purl.uniprot.org/citations/9387880 | http://purl.uniprot.org/core/volume | "49"xsd:string |