| http://purl.uniprot.org/citations/9389653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9389653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9389653 | http://www.w3.org/2000/01/rdf-schema#comment | "Genetic analysis was applied to identify novel genes involved in G protein-linked pathways controlling development. Using restriction enzyme-mediated integration (REMI), we have identified a new gene, Pianissimo (PiaA), involved in cAMP signaling in Dictyostelium discoideum. PiaA encodes a 130-kD cytosolic protein required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase. In piaA-null mutants, neither chemoattractant stimulation of intact cells nor GTPgammaS treatment of lysates activates the enzyme; constitutive expression of PiaA reverses these defects. Cytosols of wild-type cells that contain Pia protein reconstitute the GTPgammaS stimulation of adenylyl cyclase activity in piaA-lysates, indicating that Pia is directly involved in the activation. Pia and CRAC, a previously identified cytosolic regulator, are both essential for activation of the enzyme as lysates of crac- piaA-double mutants require both proteins for reconstitution. Homologs of PiaA are found in Saccharomyces cerevisiae and Schizosaccaromyces pombe; disruption of the S. cerevisiae homolog results in lethality. We propose that homologs of Pia and similar modes of regulation of these ubiquitous G protein-linked pathways are likely to exist in higher eukaryotes."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.11.23.3218"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.11.23.3218"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Chen M.-Y."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Chen M.-Y."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Devreotes P.N."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Devreotes P.N."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Long Y."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/author | "Long Y."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/pages | "3218-3231"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/pages | "3218-3231"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/title | "A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/title | "A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium."xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/9389653 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9389653 |
| http://purl.uniprot.org/citations/9389653 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9389653 |
| http://purl.uniprot.org/citations/9389653 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9389653 |
| http://purl.uniprot.org/citations/9389653 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9389653 |