| http://purl.uniprot.org/citations/9394008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9394008 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9394008 | http://www.w3.org/2000/01/rdf-schema#comment | "Human RagA and RagB is reported to be 52% identical to a putative GTPase of Saccharomyces cerevisiae, Gtr1p. According to the reported nucleotide sequence, we amplified human RagA and RagBs cDNAs from the human B cell cDNA library with PCR. Both cDNAs rescued a cold sensitivity of S. cerevisiae, gtr1-11. Furthermore, we introduced into the cloned human RagA cDNA, the mutation 'T21L' corresponding to the gtr1-11 mutation which has been reported to suppress not only all of rcc1-, temperature-sensitive mutants of Ran/Gsp1p GTPase GDP/GTP-exchanging factor, but also rna1-1, a temperature-sensitive mutant of Ran/Gsp1p GTPase-activating protein. The resulting RagAgtr1-11 cDNA partially, but significantly, suppressed both rcc1- and rna1-1 mutations. These results indicated that RagA and RagBs are functional homologues of S. cervisiae Gtr1p. Interestingly, while wild-type human RagA and RagBs were localized within the cytoplasm, similar to S. cerevisiae Gtr1p, the mutated human RagAgtr1-11 corresponding to a dominant negative form of RagA was distributed in discrete speckles in the nucleus, being localized side by side with SC-35, a non-snRNP of the splicing complex. In contrast, a dominant positive form of RagA, Q66L was localized in the cytoplasm. Thus, RagA was suggested to shuttle between the cytoplasm and the nucleus, depending on the bound nucleotide state."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.111.1.11"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.111.1.11"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Nishimoto T."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Nishimoto T."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Sekiguchi T."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Sekiguchi T."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Nakashima N."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Nakashima N."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Hirose E."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/author | "Hirose E."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/pages | "11-21"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/pages | "11-21"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/title | "RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/title | "RagA is a functional homologue of S. cerevisiae Gtr1p involved in the Ran/Gsp1-GTPase pathway."xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/volume | "111"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://purl.uniprot.org/core/volume | "111"xsd:string |
| http://purl.uniprot.org/citations/9394008 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9394008 |
| http://purl.uniprot.org/citations/9394008 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9394008 |