| http://purl.uniprot.org/citations/9396724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9396724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9396724 | http://www.w3.org/2000/01/rdf-schema#comment | "The Bcl-2 oncoprotein is a key regulator of apoptosis and the Bag-1 protein interacts with Bcl-2 and cooperates with Bcl-2 to suppress apoptosis. The human Bag-1 cDNA is essentially identical with a previously described cDNA encoding RAP46, which interacts with activated steroid hormone receptors. However, there is considerable confusion over the structure of Bag-1/RAP46 proteins and their relationship to endogenous Bag-1 proteins. Here we have characterized Bag-1 expression in mammalian cells. We demonstrate that, in addition to the previously identified 32 kDa murine and 36 kDa human Bag-1 proteins, cells express a second 50 kDa Bag-1 isoform. In some murine cell lines p50 is expressed at the same level as p32 Bag-1, and p50 and p32 Bag-1 proteins have distinct subcellular localizations, suggesting that they are functionally distinct. The published mouse Bag-1 cDNA is partial, and sequencing of additional murine Bag-1 RNA 5' sequences demonstrated that human and murine Bag-1 cDNAs contain longer open reading frames than originally suspected. We determined which open reading frames gave rise to the Bag-1 isoforms in human cells. Surprisingly, translation of neither protein initiated at the first in-frame methionine, and cells do not express Bag-1/RAP46 proteins with the previously proposed structures; p50 Bag-1 initiates at an upstream CUG codon, whereas p36 Bag-1 initiates at a downstream AUG codon. Therefore, cells express two differently localized Bag-1 isoforms generated by alternative translation initiation, and Bag-1 proteins may play a dual role in regulating apoptosis and steroid hormone-dependent transcription."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3280807"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3280807"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Packham G."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Packham G."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Cleveland J.L."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Cleveland J.L."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Brimmell M."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/author | "Brimmell M."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/pages | "807-813"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/pages | "807-813"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/title | "Mammalian cells express two differently localized Bag-1 isoforms generated by alternative translation initiation."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/title | "Mammalian cells express two differently localized Bag-1 isoforms generated by alternative translation initiation."xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/volume | "328"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://purl.uniprot.org/core/volume | "328"xsd:string |
| http://purl.uniprot.org/citations/9396724 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9396724 |
| http://purl.uniprot.org/citations/9396724 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9396724 |
| http://purl.uniprot.org/citations/9396724 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9396724 |
| http://purl.uniprot.org/citations/9396724 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9396724 |