| http://purl.uniprot.org/citations/9417100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9417100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9417100 | http://www.w3.org/2000/01/rdf-schema#comment | "Biochemical evidence suggests that the galactosyltransferase activity synthesizing type 1 carbohydrate chains is separate from the well characterized enzyme that is responsible for the synthesis of type 2 chains. This was recently confirmed by the cloning, from melanoma cells, of an enzyme capable of synthesizing type 1 chains, which was shown to have no homology to other galactosyltransferases. We report here the molecular cloning and functional expression of a second human beta3-galactosyltransferase distinct from the melanoma enzyme. The new beta3-galactosyltransferase has homology to the melanoma enzyme in the putative catalytic domain, but has longer cytoplasmic and stem regions and a carboxyl-terminal extension. Northern blots showed that the new gene is present primarily in brain and heart. When transfected into mammalian cells, this gene directs the synthesis of type 1 chains as determined by a monoclonal antibody specific for sialyl Lewisa. A soluble version of the cloned enzyme was expressed in insect cells and purified. The soluble enzyme readily catalyzes the transfer of galactose to GlcNAc to form Gal(beta1-3)GlcNAc. It also has a minor but distinct transfer activity toward Gal, LacNAc, and lactose, but is inactive toward GalNAc."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.1.433"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.1.433"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Streiff M.B."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Streiff M.B."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Katopodis A.G."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Katopodis A.G."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Kolbinger F."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/author | "Kolbinger F."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/pages | "433-440"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/pages | "433-440"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/title | "Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/title | "Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains."xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9417100 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9417100 |
| http://purl.uniprot.org/citations/9417100 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9417100 |
| http://purl.uniprot.org/citations/9417100 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9417100 |
| http://purl.uniprot.org/citations/9417100 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9417100 |