| http://purl.uniprot.org/citations/9422764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9422764 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9422764 | http://www.w3.org/2000/01/rdf-schema#comment | "Members of the erythroid Krüppel-like factor (EKLF) multigene family contain three C-terminal zinc fingers, and they are typically expressed in a limited number of tissues. EKLF, the founding member, transactivates the beta-globin promoter by binding to the CACCC motif. EKLF is essential for expression of the beta-globin gene as demonstrated by gene deletion experiments in mice. Using a DNA probe from the zinc finger region of EKLF, we cloned a cDNA encoding a member of this family from a human vascular endothelial cell cDNA library. Sequence analysis indicated that our clone, hEZF, is the human homologue of the recently reported mouse EZF and GKLF. hEZF is a single-copy gene that maps to chromosome 9q31. By gel mobility shift analysis, purified recombinant hEZF protein bound specifically to a probe containing the CACCC core sequence. In co-transfection experiments, we found that sense but not antisense hEZF decreased the activity of a reporter plasmid containing the CACCC sequence upstream of the thymidine kinase promoter by 6-fold. In contrast, EKLF increased the activity of the reporter plasmid by 3-fold. By fusing hEZF to the DNA-binding domain of GAL4, we mapped a repression domain in hEZF to amino acids 181-388. We also found that amino acids 91-117 of hEZF confer an activation function on the GAL4 DNA-binding domain."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.2.1026"xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.2.1026"xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Chin M.T."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Chin M.T."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Hsieh C.-M."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Hsieh C.-M."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Jain M.K."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Jain M.K."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Layne M.D."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Layne M.D."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Lee M.-E."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Lee M.-E."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Perrella M.A."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Perrella M.A."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Yet S.-F."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Yet S.-F."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Folta S.C."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "Folta S.C."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "McA'Nulty M.M."xsd:string |
| http://purl.uniprot.org/citations/9422764 | http://purl.uniprot.org/core/author | "McA'Nulty M.M."xsd:string |