| http://purl.uniprot.org/citations/9426000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9426000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9426000 | http://www.w3.org/2000/01/rdf-schema#comment | "Analysis of a 32.8-kb segment of DNA from the rapamycin (Rp) producer, Streptomyces hygroscopicus ATCC 29253, revealed a new type-I polyketide synthase (PKS) cluster consisting of four open reading frames (ORF 1-4), each encoding a single PKS module. The four ORFs are transcribed in the same direction and are flanked by several smaller ORFs (ORF 5-9), which may be related to the PKS cluster. The first PKS-containing ORF has a ligase domain at the N-terminus of the polypeptide. This domain has 55% aa identity to the CoA ligase domain of the Rp PKS (Schwecke et al., 1995. Proc. Natl. Acad. Sci. 92, 7839-7843) which is also encoded in this strain (Lowden et al., 1996. Angew. Chem. Int. Ed. Engl. 35, 2249-2251). ORF5 (340 aa) and ORF6 (924 aa) were found to be homologous to RapK (41% aa identity) and RapH (35% aa identity), which are hypothesized to be a pteridine-dependent dioxygenase and a regulatory protein, respectively (Molnar et al., 1996. Gene 169, 1-7). In addition, ORF7 (391 aa) was found to have up to 42% aa identity to a number of plant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases (DAHPS) and 47% aa identity to PhzF, a bacterial DAHPS involved in phenazine antibiotic synthesis. The proximity of the DAHPS-encoding gene to the PKS cluster containing a Rp-like ligase domain suggests that a derivative of shikimate may be used as the PKS starter. ORF8 (283 aa) was found to have homology (32% aa identity) to a Synechocystis sp. gene of unknown function. The N-terminal portion of ORF9 was found to be similar to a tetracycline 6-hydroxylase (34% aa identity) from Streptomyces aureofaciens."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(97)00450-2"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(97)00450-2"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.org/dc/terms/identifier | "doi:10.1016/S0378-1119(97)00450-2"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Katz L."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Katz L."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Ruan X."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Ruan X."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Stassi D."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Stassi D."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Lax S.A."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/author | "Lax S.A."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/date | "1997"xsd:gYear |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/pages | "1-9"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/pages | "1-9"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/title | "A second type-I PKS gene cluster isolated from Streptomyces hygroscopicus ATCC 29253, a rapamycin-producing strain."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/title | "A second type-I PKS gene cluster isolated from Streptomyces hygroscopicus ATCC 29253, a rapamycin-producing strain."xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/volume | "203"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://purl.uniprot.org/core/volume | "203"xsd:string |
| http://purl.uniprot.org/citations/9426000 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9426000 |