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http://purl.uniprot.org/citations/9427624http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9427624http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9427624http://www.w3.org/2000/01/rdf-schema#comment"

Background

The human major histocompatibility complex (MHC) class lb molecule HLA-E is transcribed in most tissues but little is known about its localisation within the cell. We have recently shown that HLA-E binds signal-sequence-derived peptides from human MHC class I molecules in vitro.

Results

Using a newly characterised antibody recognising HLA-E, we show that HLA-E is expressed at the cell surface. We demonstrate that HLA-E surface expression is correlated with the presence of MHC class I molecules which provide suitable leader sequence peptides capable of binding to HLA-E. Further studies on the interaction of HLA-E with molecules in the endoplasmic reticulum revealed that HLA-E associates with the transporter associated with antigen processing (TAP) and calreticulin, and that HLA-E expression is TAP-dependent and tapasin-dependent. In addition, HLA-E dissociates from TAP upon binding of MHC class I leader sequence peptides.

Conclusion

These experiments establish that surface expression of HLA-E is regulated by the binding of a restricted pool of peptides from the leader sequence of MHC class I molecules. The correlation between HLA-E and MHC class I surface expression might be relevant to the function of HLA-E. Our results also show that, although these HLA-E binding peptides are derived from signal sequences, they may be released back into the cytosol and subsequently translocated by the TAP complex and loaded onto HLA-E molecules."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.org/dc/terms/identifier"doi:10.1016/s0960-9822(98)70014-4"xsd:string
http://purl.uniprot.org/citations/9427624http://purl.org/dc/terms/identifier"doi:10.1016/s0960-9822(98)70014-4"xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Wilson D."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Wilson D."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"McMichael A.J."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"McMichael A.J."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Allan D.S."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Allan D.S."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Braud V.M."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/author"Braud V.M."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/pages"1-10"xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/pages"1-10"xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/title"TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/title"TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide."xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/9427624http://purl.uniprot.org/core/volume"8"xsd:string
http://purl.uniprot.org/citations/9427624http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9427624
http://purl.uniprot.org/citations/9427624http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9427624
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