| http://purl.uniprot.org/citations/9430691 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9430691 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9430691 | http://www.w3.org/2000/01/rdf-schema#comment | "In excitable cells, intracellular Ca2+ is released via the ryanodine receptor from the intracellular Ca2+ storing structure, the sarcoplasmic reticulum. To determine whether this released Ca2+ propagates through gap junctions to neighboring cells and thereby constitutes a long range signaling network, we developed a cell system in which cells expressing both connexin-43 and ryanodine receptor are surrounded by cells expressing only connexin-43. When the ryanodine receptor in cells was activated by caffeine, propagation of Ca2+ from these caffeine-responsive cells to neighboring cells was observed with a Ca2+ imaging system using fura-2/AM. Inhibitors of gap junctional communication rapidly and reversibly abolished this propagation of Ca2+. Together with the electrophysiological analysis of transfected cells, the observed intercellular Ca2+ wave was revealed to be due to the reconstituted gap junction of transfected cells. We next evaluated the functional roles of cysteine residues in the extracellular loops of connexin-43 in gap junctional communication. Mutations of Cys54, Cys187, Cys192, and Cys198 to Ser showed the failure of Ca2+ propagation to neighboring cells in accordance with the electrical uncoupling between transfected cells, whereas mutations of Cys61 and Cys68 to Ser showed the same pattern as the wild type. [14C]Iodoacetamide labeling of free thiols of cysteine residues in mutant connexin-43s showed that two pairs of intramolecular disulfide bonds are formed between Cys54 and Cys192 and between Cys187 and Cys198. These results suggest that intercellular Ca2+ signaling takes place in cultured cells expressing connexin-43, leading to their own synchronization and that the extracellular disulfide bonds of connexin-43 are crucial for this process."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.3.1519"xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.3.1519"xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Hori M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Hori M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Otsu K."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Otsu K."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Yabuki M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Yabuki M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Tada M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Tada M."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Toyofuku T."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Toyofuku T."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Kuzuya T."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/author | "Kuzuya T."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/pages | "1519-1528"xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/pages | "1519-1528"xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/title | "Intercellular calcium signaling via gap junction in connexin-43-transfected cells."xsd:string |
| http://purl.uniprot.org/citations/9430691 | http://purl.uniprot.org/core/title | "Intercellular calcium signaling via gap junction in connexin-43-transfected cells."xsd:string |