| http://purl.uniprot.org/citations/9442054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9442054 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9442054 | http://www.w3.org/2000/01/rdf-schema#comment | "The DNA-dependent protein kinase (DNA-PK) is composed of a large catalytic subunit of approximately 470 kDa (DNA-PKcs) and the DNA-binding protein, Ku. Absence of DNA-PK activity confers sensitivity to x-rays and defects in both DNA double-strand break repair and V(D)J recombination. However the precise function of DNA-PK in DNA double-strand break repair is not known. Here we show, using electrophoretic mobility shift assays, that polypeptides in a fraction purified from human cells interact with DNA-PK and stabilize the formation of a complex containing DNA-PKcs-Ku and DNA. Five polypeptides in this fraction have been identified by amino-terminal sequence analysis and/or immunoblotting. These proteins are NF90 and NF45, which are the 90- and 45-kDa subunits of a protein known to bind specifically to the antigen receptor response element of the interleukin 2 promoter, and the alpha, beta, and gamma subunits of eukaryotic translation initiation factor eIF-2. We also show that NF90, NF45, and eIF-2 beta are substrates for DNA-PK in vitro. In addition, recombinant NF90 promotes formation of a complex between DNA-PKcs, Ku, and DNA, and antibodies to recombinant NF90 or recombinant NF45 immunoprecipitate DNA-PKcs in vitro. Together, our data suggest that NF90, in complex with NF45, interacts with DNA-PKcs and Ku on DNA and that NF90 and NF45 may be important for the function of DNA-PK."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.4.2136"xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.4.2136"xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Kao P.N."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Kao P.N."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Chan D.W."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Chan D.W."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Lees-Miller S.P."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Lees-Miller S.P."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Ting N.S.Y."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Ting N.S.Y."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Lintott L.G."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/author | "Lintott L.G."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/pages | "2136-2145"xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/pages | "2136-2145"xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/title | "DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/title | "DNA-dependent protein kinase interacts with antigen receptor response element binding proteins NF90 and NF45."xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9442054 | http://purl.uniprot.org/core/volume | "273"xsd:string |