| http://purl.uniprot.org/citations/9463369 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9463369 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9463369 | http://www.w3.org/2000/01/rdf-schema#comment | "Replication of human immunodeficiency virus type 1 (HIV-1) in non-dividing cells critically depends on import of the viral pre-integration complex into the nucleus. Genetic evidence suggests that viral protein R (Vpr) and matrix antigen (MA) are directly involved in the import process. An in vitro assay that reconstitutes nuclear import of HIV-1 pre-integration complexes in digitonin-permeabilized cells was used to demonstrate that Vpr is the key regulator of the viral nuclear import process. Mutant HIV-1 pre-integration complexes that lack Vpr failed to be imported in vitro, whereas mutants that lack a functional MA nuclear localization sequence (NLS) were only partially defective. Strikingly, the import defect of the Vpr-mutant was rescued when recombinant Vpr was re-added. In addition, import of Vpr-virus was rescued by adding the cytosol of HeLa cells, where HIV-1 replication had been shown to be Vpr-independent. In a solution binding assay, Vpr associated with karyopherin alpha, a cellular receptor for NLSs. This association increased the affinity of karyopherin alpha for basic-type NLSs, including that of MA, thus explaining the positive effect of Vpr on nuclear import of the HIV-1 pre-integration complex and BSA-NLS conjugates. These results identify the biochemical mechanism of Vpr function in transport of the viral pre-integration complex to, and across, the nuclear membrane."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.4.909"xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.4.909"xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Ratner L."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Ratner L."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Blobel G."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Blobel G."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Lee M.A."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Lee M.A."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Popov S."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Popov S."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Bukrinsky M."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Bukrinsky M."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Zybarth G."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Zybarth G."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Lane C.M."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Lane C.M."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Moore M.S."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Moore M.S."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Reiling N."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Reiling N."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Rexach M."xsd:string |
| http://purl.uniprot.org/citations/9463369 | http://purl.uniprot.org/core/author | "Rexach M."xsd:string |