| http://purl.uniprot.org/citations/9463377 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9463377 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9463377 | http://www.w3.org/2000/01/rdf-schema#comment | "We isolated a novel yeast alpha-COP mutant, ret1-3, in which alpha-COP is degraded after cells are shifted to a restrictive temperature. ret1-3 cells cease growth at 28 degrees C and accumulate the ER precursor of carboxypeptidase Y (p1 CPY). In a screen for high copy suppressors of these defects, we isolated the previously unidentified yeast epsilon-COP gene. epsilon-COP (Sec28p) overproduction suppresses the defects of ret1-3 cells up to 34 degrees C, through stabilizing levels of alpha-COP. Surprisingly, cells lacking epsilon-COP (sec28 Delta) grow well up to 34 degrees C and display normal trafficking of carboxypeptidase Y and KKXX-tagged proteins at a permissive temperature. epsilon-COP is thus non-essential for yeast cell growth, but sec28 Delta cells are thermosensitive. In sec28 Delta cells shifted to 37 degrees C, wild-type alpha-COP (Ret1p) levels diminish rapidly and cells accumulate p1 CPY; these defects can be suppressed by alpha-COP overproduction. Mutant coatomer from sec28 Delta cells behaves as an unusually large protein complex in gel filtration experiments. The sec28 Delta mutation displays allele-specific synthetic-lethal interactions with alpha-COP mutations: sec28 Delta ret1-3 double mutants are unviable at all temperatures, whereas sec28 Delta ret1-1 double mutants grow well up to 30 degrees C. Our results suggest that a function of epsilon-COP is to stabilize alpha-COP and the coatomer complex."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.4.985"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.4.985"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Schekman R."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Schekman R."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Duden R."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Duden R."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Kajikawa L."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Kajikawa L."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Wuestehube L."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/author | "Wuestehube L."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/pages | "985-995"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/pages | "985-995"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/title | "Epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/title | "Epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP."xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/9463377 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9463377 |
| http://purl.uniprot.org/citations/9463377 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9463377 |