http://purl.uniprot.org/citations/9469821 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9469821 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9469821 | http://www.w3.org/2000/01/rdf-schema#comment | "Polypeptides remaining tightly associated with isolated genomic DNA are of interest with respect to their potential involvement in the topological organization and/or function of genomic DNA. Such residual DNA-polypeptide complexes were used for raising monoclonal antibodies by in vitro immunization. Screening of a murine lambdagt11 cDNA library with these antibodies released a positive cDNA (MC1D) encoding a 16 kDa polypeptide. The cloned homologous human cDNA (HC1D) was identified in the dbest data base by partial sequence comparison, and it was sequenced full length. The cDNA-derived amino acid sequences comprise nuclear location signals but none of the known DNA-binding motifs. However, the recombinantly expressed proteins show in vitro DNA binding affinities. A polyclonal antiserum to the recombinant MC1D protein immunostains sub-nuclear structures, and it detects a residual 16 kDa polypeptide on western blots of DNA digests. These results support the conclusion that the cloned cDNAs reflect mRNAs encoding one of the chemically-resistant polypeptides which can be detected in isolated genomic DNA by sensitive techniques, e.g. by125Iodine labeling and SDS-PAGE."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/26.5.1160"xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/26.5.1160"xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Werner D."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Werner D."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Spiess E."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Spiess E."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Glaser T."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Glaser T."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Greferath R."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Greferath R."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Keck T."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Keck T."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Nehls P."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Nehls P."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Rothbarth K."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Rothbarth K."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Stammer H."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/author | "Stammer H."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |
http://purl.uniprot.org/citations/9469821 | http://purl.uniprot.org/core/name | "Nucleic Acids Res."xsd:string |