| http://purl.uniprot.org/citations/9472031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9472031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9472031 | http://www.w3.org/2000/01/rdf-schema#comment | "Dynamins are 100-kilodalton guanosine triphosphatases that participate in the formation of nascent vesicles during endocytosis. Here, we have tested if novel dynamin-like proteins are expressed in mammalian cells to support vesicle trafficking processes at cytoplasmic sites distinct from the plasma membrane. Immunological and molecular biological methods were used to isolate a cDNA clone encoding an 80-kilodalton novel dynamin-like protein, DLP1, that shares up to 42% homology with other dynamin-related proteins. DLP1 is expressed in all tissues examined and contains two alternatively spliced regions that are differentially expressed in a tissue-specific manner. DLP1 is enriched in subcellular membrane fractions of cytoplasmic vesicles and endoplasmic reticulum. Morphological studies of DLP1 in cultured cells using either a specific antibody or an expressed green fluorescent protein (GFP)-DLP1 fusion protein revealed that DLP1 associates with punctate cytoplasmic vesicles that do not colocalize with conventional dynamin, clathrin, or endocytic ligands. Remarkably, DLP1-positive structures coalign with microtubules and, most strikingly, with endoplasmic reticulum tubules as verified by double labeling with antibodies to calnexin and Rab1 as well as by immunoelectron microscopy. These observations provide the first evidence that a novel dynamin-like protein is expressed in mammalian cells where it associates with a secretory, rather than endocytic membrane compartment."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.140.4.779"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.140.4.779"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "McNiven M.A."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "McNiven M.A."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Yoon Y."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Yoon Y."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Dahan S."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Dahan S."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Pitts K.R."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/author | "Pitts K.R."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/pages | "779-793"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/pages | "779-793"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/title | "A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/title | "A novel dynamin-like protein associates with cytoplasmic vesicles and tubules of the endoplasmic reticulum in mammalian cells."xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/volume | "140"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://purl.uniprot.org/core/volume | "140"xsd:string |
| http://purl.uniprot.org/citations/9472031 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9472031 |
| http://purl.uniprot.org/citations/9472031 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9472031 |