| http://purl.uniprot.org/citations/9486191 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9486191 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9486191 | http://www.w3.org/2000/01/rdf-schema#comment | "It has been proposed that the hepatocellular Na(+)-dependent bile salt uptake system exhibits a broad substrate specificity in intact hepatocytes. In contrast, recent expression studies in mammalian cell lines have suggested that the cloned rat liver Na(+)-taurocholate cotransporting polypeptide (Ntcp) may transport only taurocholate. To characterize its substrate specificity Ntcp was stably transfected into Chinese hamster ovary (CHO) cells. These cells exhibited saturable Na(+)-dependent uptake of [3H]taurocholate [Michaelis constant (K(m)) of approximately 34 microM] that was strongly inhibited by all major bile salts, estrone 3-sulfate, bumetanide, and cyclosporin A. Ntcp cRNA-injected Xenopus laevis oocytes and the transfected CHO cells exhibited saturable Na(+)-dependent uptake of [3H]taurochenodeoxycholate (Km of approximately 5 microM), [3H]tauroursodeoxycholate (Km of approximately 14 microM), and [14C]glycocholate (Km of approximately 27 microM). After induction of gene expression by sodium butyrate, Na(+)-dependent transport of [3H]estrone 3-sulfate (Km of approximately 27 microM) could also be detected in the transfected CHO cells. However, there was no detectable Na(+)-dependent uptake of [3H]bumetanide or [3H]cyclosporin A. These results show that the cloned Ntcp can mediate Na(+)-dependent uptake of all physiological bile salts as well as of the steroid conjugate estrone 3-sulfate. Hence, Ntcp is a multispecific transporter with preference for bile salts and other anionic steroidal compounds."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.1998.274.2.g370"xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.1998.274.2.g370"xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Hagenbuch B."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Hagenbuch B."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Hofmann A.F."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Hofmann A.F."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Meier P.J."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Meier P.J."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Stieger B."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Stieger B."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Schroeder A."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Schroeder A."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Eckhardt U."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Eckhardt U."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Schteingart C.D."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Schteingart C.D."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Tynes R."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/author | "Tynes R."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |
| http://purl.uniprot.org/citations/9486191 | http://purl.uniprot.org/core/name | "Am. J. Physiol."xsd:string |