| http://purl.uniprot.org/citations/9488445 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9488445 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9488445 | http://www.w3.org/2000/01/rdf-schema#comment | "We report the characterization of clone 1.9.2, a gene expressed in mineralizing osteoblasts. Remarkably, clone 1.9.2 is the murine homolog of the alpha chain of the nascent polypeptide-associated complex (alpha-NAC). Based on sequence similarities between alpha-NAC/1.9.2 and transcriptional regulatory proteins and the fact that the heterodimerization partner of alpha-NAC was identified as the transcription factor BTF3b (B. Wiedmann, H. Sakai, T. A. Davis, and M. Wiedmann, Nature 370:434-440, 1994), we investigated a putative role for alpha-NAC/ 1.9.2 in transcriptional control. The alpha-NAC/1.9.2 protein potentiated by 10-fold the activity of the chimeric activator GAL4/VP-16 in vivo. The potentiation was shown to be mediated at the level of gene transcription, because alpha-NAC/1.9.2 increased GAL4/VP-16-mediated mRNA synthesis without affecting the half-life of the GAL4/VP-16 fusion protein. Moreover, the interaction of alpha-NAC/1.9.2 with a transcriptionally defective mutant of GAL4/VP-16 was severely compromised. Specific protein-protein interactions between alpha-NAC/1.9.2 and GAL4/VP-16 were demonstrated by gel retardation, affinity chromatography, and protein blotting assays, while interactions with TATA box-binding protein (TBP) were detected by immunoprecipitation, affinity chromatography, and protein blotting assays. Based on these interactions that define the coactivator class of proteins, we conclude that the alapha-NAC/1.9.2 gene product functions as a transcriptional coactivator."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.18.3.1303"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.18.3.1303"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "Moreau A."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "Moreau A."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "Yotov W.V."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "Yotov W.V."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "St Arnaud R."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/author | "St Arnaud R."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/pages | "1303-1311"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/pages | "1303-1311"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/title | "The alpha chain of the nascent polypeptide-associated complex functions as a transcriptional coactivator."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/title | "The alpha chain of the nascent polypeptide-associated complex functions as a transcriptional coactivator."xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://purl.uniprot.org/core/volume | "18"xsd:string |
| http://purl.uniprot.org/citations/9488445 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9488445 |
| http://purl.uniprot.org/citations/9488445 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9488445 |
| http://purl.uniprot.org/citations/9488445 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9488445 |
| http://purl.uniprot.org/citations/9488445 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9488445 |