| http://purl.uniprot.org/citations/9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9490675 | http://www.w3.org/2000/01/rdf-schema#comment | "The membrane-distal region of the cytoplasmic domain of human granulocyte colony-stimulating factor receptor (G-CSF-R) contains four conserved tyrosine residues: Y704, Y729, Y744, and Y764. Three of these (Y729, Y744, and Y764) are located in the C-terminal part of G-CSF-R, previously shown to be essential for induction of neutrophilic differentiation. To determine the role of the tyrosines in G-CSF-mediated responses, we constructed tyrosine-to-phenylalanine (Y-to-F) substitution mutants and expressed these in a differentiation competent subclone of 32D cells that lacks endogenous G-CSF-R. We show that all tyrosines can be substituted essentially without affecting the differentiation signaling properties of G-CSF-R. However, substitution of one specific tyrosine, ie, Y764, markedly influenced proliferation signaling as well as the timing of differentiation. 32D cells expressing wild-type (WT) G-CSF-R (or mutants Y704F, Y729F, or Y744F) proliferated in G-CSF-containing cultures until day 8 and then developed into mature neutrophils. In contrast, 32D/Y764F cells arrested in the G1 phase of the cell cycle within 24 hours and showed complete neutrophilic differentiation after 3 days of culture. This resulted in an average 30-fold reduction of neutrophil production as compared with the 32D/WT controls. Importantly, G-CSF-mediated activation of Shc, p21Ras and the induction of c-myc were severely reduced by substitution of Y764. These findings indicate that Y764 of G-CSF-R is crucial for maintaining the proliferation/differentiation balance during G-CSF-driven neutrophil development and suggest a role for multiple signaling mechanisms in maintaining this balance."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Bos J.L."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "van Leeuwen D."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Smith L."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Burgering B.M."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Lowenberg B."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Schelen A.M."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Touw I.P."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "de Koning J.P."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Santini V."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/author | "Soede-Bobok A.A."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/pages | "1924-1933"xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/title | "Proliferation signaling and activation of Shc, p21Ras, and Myc via tyrosine 764 of human granulocyte colony-stimulating factor receptor."xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://purl.uniprot.org/core/volume | "91"xsd:string |
| http://purl.uniprot.org/citations/9490675 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9490675 |
| http://purl.uniprot.org/citations/9490675 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9490675 |
| http://purl.uniprot.org/uniprot/#_P09919-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |
| http://purl.uniprot.org/uniprot/#_O60674-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |
| http://purl.uniprot.org/uniprot/#_P07948-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |
| http://purl.uniprot.org/uniprot/#_P08631-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |
| http://purl.uniprot.org/uniprot/#_P29597-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |
| http://purl.uniprot.org/uniprot/#_Q06124-mappedCitation-9490675 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9490675 |