| http://purl.uniprot.org/citations/9506957 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9506957 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9506957 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9506957 | http://www.w3.org/2000/01/rdf-schema#comment | "We isolated a cDNA encoding a novel glucuronyltransferase from human placenta cDNA with the use of the degenerate reverse transcriptase-polymerase chain reaction method. Degenerate primers were designed based upon the amino acid sequence alignment of rat glucuronyltransferase (GlcAT-P) involved in the biosynthesis of the carbohydrate epitope HNK-1 with putative proteins in Caenorhabditis elegans and Schistosoma mansoni. The new cDNA sequence revealed an open reading frame coding for a protein of 335 amino acids with a type II transmembrane protein topology. The amino acid sequence displayed 43% identity to the rat GlcAT-P, and the highest sequence identity was found in the COOH-terminal catalytic domain. The expression of a soluble recombinant form of the protein in COS-1 cells produced an active glucuronyltransferase with marked specificity for a glycoserine Galbeta1-3Galbeta1-4Xylbeta1-O-Ser. In contrast, asialoorosomucoid, which contains the Galbeta1-4GlcNAc sequence and is a good acceptor substrate for the GlcAT-P, did not serve as an acceptor. The reaction product was sensitive to beta-glucuronidase digestion and co-chromatographed with authentic GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser in high-performance liquid chromatography, suggesting that the enzyme is a beta1, 3-glucuronyltransferase. These results indicate that this new member of the glucuronyltransferase gene family is the enzyme previously described as glucuronyltransferase I that forms the glycosaminoglycan-protein linkage region, GlcAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser, of proteoglycans."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.12.6615"xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.12.6615"xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Kitagawa H."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Kitagawa H."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Ogawa T."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Ogawa T."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Sugahara K."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Sugahara K."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Kawasaki T."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Kawasaki T."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Oka S."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Oka S."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Tone Y."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Tone Y."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Neumann K.W."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Neumann K.W."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Tamura J."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/author | "Tamura J."xsd:string |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9506957 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |